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Foundations in Microbiology, 4/e
Kathleen Park Talaro, Pasadena City College
Arthur Talaro

Microbial Genetics

Supplemental Quiz

1. The most likely reason that mRNA is the least abundant species of total cellular RNA is?

A. There are fewer genes for mRNA.
B. mRNA is readily degraded once it is made.
C. mRNA binds to single stranded binding proteins and is not detectable.
D. There are fewer RNA polymerases which transcribe mRNA compared with tRNA and rRNA.
E. Transcription of mRNA occurs after tRNA and rRNA using whatever nucleosides are left.

2. What characteristic(s) of RNA result in their ability to bind to each other? How does binding of RNA direct protein synthesis? Design a novel antibiotic that would have a method of action related to protein synthesis inhibition based on this concept.

3. Explain how nonlethal mutations produce more fit members of the species.

4. Design an experiment that would produce mutant bacteria that are better able to survive acid rain drainage. Next, design a plasmid containing the genes coding for products required above and have them controlled by a "lac operon-like" promoter. What regulates gene expression?