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Multiple Choice Quiz
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1

Using genetic engineering genes can be
A)moved from one organism to potentially any other organism.
B)easily converted to produce any product we desire.
C)turned off or on at our whim.
D)used to cure all known diseases.
E)create plants resistant to all insect pests.
2

Genetic engineering has produced salmon
A)with shorter reproductive cycles.
B)that produce four times more offspring.
C)resistant to viral infection.
D)that are heavier than non-engineered salmon.
E)both a and d
3

Sticky ends are the result of
A)treatment of a nucleotide sequence with DNA ligase.
B)exposure of eukaryotic DNA to a prokaryotic plasmid.
C)infection of a cell with a bacteriophage.
D)DNA breaking down in the presence of reverse transcriptase.
E)cutting by restriction enzymes "off center" in a specific nucleotide sequence.
4

In order to produce a DNA fragment that can be inserted into the DNA of a second organism one would need to
A)create a compatible DNA segment from an mRNA template.
B)find a virus that could serve as a vector for transfer of the donor DNA.
C)make sure both organisms have compatible DNA.
D)be sure both donor and recipient DNA were prokaryotic or eukaryotic.
E)cut the DNA of the donor and recipient cells with the same restriction enzyme.
5

Sanger earned his second Nobel Prize for development of a technique called DNA fingerprinting.
A)true
B)false
6

Cloned human genes are localized by using radioactive probes to detect binding between the probe and the complementary sequence on the chromosome.
A)true
B)false
7

Interleukins:
A)stimulate white cell production.
B)activate white blood cells. Used to treat HIV and cancer.
C)halt the production of viruses.
D)used to treat individuals with kidney disorders.
8

Erythropoietin:
A)stimulates white cell production.
B)activates white blood cells. Used to treat HIV and cancer.
C)halts the production of viruses.
D)used to treat individuals with kidney disorders.
9

Interferons:
A)stimulate white cell production.
B)activate white blood cells. Used to treat HIV and cancer.
C)halt the production of viruses.
D)used to treat individuals with kidney disorders.
10

Colony-stimulating factors:
A)stimulate white cell production.
B)activate white blood cells. Used to treat HIV and cancer.
C)halt the production of viruses.
D)used to treat individuals with kidney disorders.
11

Genes can be inserted into cells by
A)shooting them into the cell with a DNA particle gun.
B)using a plasmid as a gene vector.
C)causing them to be produced in the recipient cell.
D)all of the above
E)only a and b
12

Glyphosate prevents plants from growing by
A)cleaving critical DNA in the plant and halting cell division.
B)preventing the production of gametes.
C)preventing the formation of spindle fibers and halting mitosis.
D)stopping them from synthesizing proteins.
E)both a and d.
13

One possible risk of genetic engineering is the accidental production of dangerously resistant microorganisms.
A)true
B)false
14

Although risks are possible, gene engineering appears to offer more of a contribution to human welfare than threat.
A)true
B)false
15

You are conducting research on eight species of Tribolium flour beetles and you want to compare their proteins. Which of the following techniques might you use?
A)genetic engineering
B)gene amplification
C)gel electrophoresis
D)polymerase chain reaction
E)gene therapy
16

The key to isolating a particular gene for use in recombinant DNA research is to
A)find a restriction enzyme to cut out the gene.
B)find a compatible species to receive the gene.
C)identify copies of the gene on several chromosomes.
D)both a and b
E)none of the above
17

Cuts in DNA are sealed with
A)restriction enzymes.
B)ligases.
C)reverse transcriptase.
D)polymerase.
E)plasmids.
18

DNA is made from RNA with the help of
A)restriction enzymes.
B)ligases.
C)reverse transcriptase.
D)polymerase.
E)plasmids.
19

Sticky ends are created by
A)adding ligase to the end of the DNA fragment.
B)adding polymerase to the end of the DNA fragment.
C)cutting DNA so that there are short, single strands at each end.
D)cutting the DNA fragment in several places.
E)both a and d.
20

When investigating a crime scene detectives find a single hair on the floor. The sample is not large enough to perform many tests. Which of the following methods could be used to make sufficient DNA to allow for comparison to the DNA of the prime suspect?
A)Reverse transcriptase could be used to make DNA from RNA in the hair sample.
B)The hair root could be tissue cultured to make additional copies of the DNA.
C)The gene could be amplified by use of electrophoresis.
D)Polymerase chain reaction could be used to make many copies of the DNA.
E)The gene of interest could be spliced into a bacterium that would make a new copy every time it replicated.
21

cDNA is significant when
A)you need several thousand copies of a gene.
B)cloning eukaryotic DNA in prokaryotic cells.
C)it is necessary to copy introns in prokaryotic cells.
D)a primary transcript is not available.
E)processed mRNA is not available.
22

In order for plants to resist the effects of the herbicide Roundup, they must be
A)monocots.
B)dicots.
C)annuals.
D)able to synthesize glyphosate.
E)resistant to glyphosate.
23

Although the Ti plasmid has revolutionized plant genetic engineering, one limitation of its use is that it
A)is only about 50% effective.
B)can only be used on fruit-bearing plants.
C)is only effective on plants that are resistant to Roundup.
D)does not infect cereal plants.
E)cannot transmit prokaryotic genes.





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