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Multiple Choice Quiz
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1

Cutting certain genes out of molecules of DNA requires the use of special
A)degrading nucleases.
B)restriction endonucleases.
C)eukaryotic enzymes.
D)viral enzymes.
2

Which of the following cannot be used as a vector?
A)phage
B)plasmid
C)bacterium
D)All can be used as vectors.
3

In Cohen and Boyer's recombinant DNA experiments, restriction endonucleases were used to
A)isolate fragments of cloned bacterial plasmids.
B)isolate fragments of frog DNA that contained an rRNA gene.
C)cleave the bacterial plasmid.
D)All of these are correct.
4

A DNA library is
A)a general collection of all genes sequenced thus far.
B)a collection of DNA fragments that make up the entire genome of a particular organism.
C)a DNA fragment inserted into a vector.
D)all DNA fragments identified with a probe.
5

A probe is used in which stage of genetic engineering?
A)cleaving DNA
B)recombining DNA
C)cloning
D)screening
6

The enzyme used in the polymerase chain reaction is
A)restriction endonuclease.
B)reverse transcriptase.
C)DNA polymerase.
D)RNA polymerase.
7

A method used to distinguish DNA of one individual from another is
A)polymerase chain reaction.
B)cDNA.
C)reverse transcriptase.
D)restriction fragment length polymorphism.
8

Inserting a gene encoding a pathogenic microbe's surface protein into a harmless virus produces a
A)piggyback vaccine.
B)virulent virus.
C)active disease-causing pathogen.
D)pharmaceutical human protein.
9

Although the Ti plasmid has revolutionized plant genetic engineering, one limitation of its use is that it
A)cannot infect broadleaf plants.
B)cannot be used on fruit-bearing plants.
C)cannot transmit prokaryotic genes.
D)does not infect cereal plants such as corn and rice.
10

Which of the following is not an application of genetic engineering in plants?
A)nitrogen fixation
B)DNA vaccines
C)resistance to glyphosate
D)production of insecticidal proteins in plants







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