Review the animation and answer the questions that follow to test your understanding.

1			The purpose of PCR is to
			A)	make more copies of DNA primers to increase protein synthesis
			B)	make many copies of an organism’s DNA sequence so a small number of organisms will become large enough to be identified
			C)	make more RNA so large units of protein can be synthesized
			D)	recycle DNA using thermocyclers
2			For DNA amplification to occur, which of the following are needed?
			A)	loose ribonucleotides
			B)	RNA primers
			C)	thermostable DNA polymerase
			D)	b and c
			E)	all of the above
3			Taq polymerase starts copying at
			A)	the end of free single-stranded RNA
			B)	any open point
			C)	RNA primers attached to the end of the desired gene
			D)	DNA primers attached to the end of the desired gene
4			Using PCR, over 1 million copies of DNA can be made from a single strand of DNA overnight.
			A)	True
			B)	False
5			When DNA is heated, primers anneal to DNA strands.
			A)	True
			B)	False