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Tweaking the Experiment
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First Experiment



1

If a mated strain grew on plates containing azide, lactose, galactose, and formed T1 plaques, its genotype would be
A)azides, T1s, lac+, gal+
B)azider, T1s, lac+, gal+
C)azides, T1s, lac-, gal-
D)azider, T1r, lac-, gal-
2

Based on the gene order described in this experiment, if a mated strain was T1 resistant, it would also be
A)able to grow on lactose and galactose
B)able to grow on lactose but unable to grow on galactose
C)unable to grow on lactose but able to grow on galactose
D)unable to grow on lactose and galactose
3

The purpose of the blender treatment is
A)To promote the binding between the two strains
B)To promote the transfer of DNA between the two strains
C)To separate the mated cells
D)To promote recombination of the donor DNA into the recipient chromosome
4

The reason why it took so long for the gal+ gene to be transferred was because
A)The gal+ gene was farthest from the origin of transfer.
B)The gal+ gene is the largest gene.
C)The gal+ gene does not readily recombine into the recipient chromosome.
D)The gal+ gene is susceptible to mutation.
5

The reason why the researchers did not observe mated cells that were streptomycin sensitive was because
A)The strs gene in the donor strain was too far from the origin of transfer.
B)The cells were initially placed on plates with streptomycin, which would kill streptomycin-sensitive cells.
C)The gene is too large to be transferred.
D)None of the above.







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