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| 1 | |
Which has a longer-lived effect on a cell, a change in genotype or a change
in phenotype? Explain. |
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| 2 | |
Would the addition of three bases to DNA always lead to a frameshift mutation?
Explain. |
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| 3 | |
If you wished to isolate a point mutant, what mutagen would you use? To
isolate a knockout mutant? |
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| 4 | |
If you wish to maximize the number of mutations following UV irradiation,
should you incubate the irradiated cells in the light or in the dark, or does
it make any difference? Explain your answer. |
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| 5 | |
Is it as effective to take two antibiotics sequentially as it is to take
them simultaneously, as long as the total length of time that they are both
taken is the same? Explain. |
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| 6 | |
The Ames test measures the reversion of a mutant gene. Would it be just
as good a test for the generation of a mutant gene rather than its reversion?
Explain. |
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| 7 | |
How could you demonstrate by replica plating that the environment selects
but does not mutate genes in bacteria? |
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| 8 | |
Could you devise a test using DNA-mediated transformation that could test
chemicals for their mutagenic activity? |
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| 9 | |
If gene 1 is on the chromosome and gene 2 is on a plasmid, which one would
be transduced at a higher frequency? Explain. |
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| 10 | |
Would you expect conjugation to be more efficient if cells were plated
together on solid medium (agar) or mixed together in a liquid in a shaking flask?
Explain. |
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| 11 | |
What happens to chromosomal DNA that is transferred in conjugation if
it does not integrate into the recipient cell's chromosome? Explain. |
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| 12 | |
What does the phrase "reservoir for R plasmids" mean when referring to
plasmids carried by non-disease-causing bacteria? |
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| 13 | |
Do you think it would be easier to cure a bacterium containing high-
or low-copy-number plasmids? Explain. |
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| 14 | |
Why should a pathogenicity island contain several virulence factors and
not just one? |
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| 15 | |
What do you think is the substrate for transposase enqyme? |
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| 16 | |
What effect does a lack of a restriction-modification system have on the
entry of foreign DNA? |
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| 17 | |
What is the most likely mechanism for the transfer of antibiotic resistance
from E. coli to Clostridium? Why? |
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