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Molecular Biology, 4/e
Robert F. Weaver

Molecular Cloning Methods

Polymerase Chain Reaction

View the animation below, then complete the quiz to test your knowledge of the concept.



1

PCR requires all of the following EXCEPT
A)primers.
B)DNA ligase.
C)DNA polymerase.
D)DNA of interest.
E)deoxyrobinucleotides.
2

If you start with one double-stranded DNA molecule and you perform SIX cycles of PCR, how many double-stranded copies of the DNA will you have?
A)6
B)8
C)16
D)32
E)64
3

The most likely source of the Taq polymerase used in PCR is a bacterium that lives in
A)soil.
B)arctic ice.
C)hot vents.
D)humans.
E)plants.
4

PCR can be used to amplify DNA from any source.
A)True
B)False
5

During the PCR, the hydrogen bonds of the double-stranded DNA molecules are broken by the enzyme helicase.
A)True
B)False