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Microbiology, Fifth Edition
Microbiology, 5/e
Lansing M Prescott, Augustana College
Donald A Klein, Colorado State University
John P Harley, Eastern Kentucky University
Metabolism: The Use of Energy in Biosynthesis

Multiple Choice Quiz

Choose the best answer



In the next set of questions we are concerned with the light and dark reactions of photosynthesis. The light reactions are to be considered reaction group I. The enzymatic reactions are to be considered group II. Categorize the next set of questions according to the following keys.



1

Oxidation
A)a reaction of I but not II.
B)a reaction of II but not I.
C)a reaction of both I and II.
D)not a reaction of either I or II but necessary if either I or II or both are to occur.
E)not a reaction of either I or II but occurs as a result of I or II or both.
2

Photophosphorylation
A)a reaction of I but not II.
B)a reaction of II but not I.
C)a reaction of both I and II.
D)not a reaction of either I or II but necessary if either I or II or both are to occur.
E)not a reaction of either I or II but occurs as a result of I or II or both.
3

Production of photons
A)a reaction of I but not II.
B)a reaction of II but not I.
C)a reaction of both I and II.
D)not a reaction of either I or II but necessary if either I or II or both are to occur.
E)not a reaction of either I or II but occurs as a result of I or II or both.
4

Production of a carbohydrate
A)a reaction of I but not II.
B)a reaction of II but not I.
C)a reaction of both I and II.
D)not a reaction of either I or II but necessary if either I or II or both are to occur.
E)not a reaction of either I or II but occurs as a result of I or II or both.
5

Production of O2
A)a reaction of I but not II.
B)a reaction of II but not I.
C)a reaction of both I and II.
D)not a reaction of either I or II but necessary if either I or II or both are to occur.
E)not a reaction of either I or II but occurs as a result of I or II or both.
6

If you could label the oxygen in CO2 and provide this CO2 to a plant, where would you expect to find this labeled oxygen after the plant had undergone photosynthesis?
A)in the water used
B)in the NADPH
C)in the carbohydrate produced
D)in the oxygen given off by the plant
7

Which one of the following is a product of both cyclic and noncyclic photophosphorylation?
A)NADPH
B)O2
C)H2O
D)ATP
E)Carbohydrate
8

In noncyclic photophosphorylation, the ultimate acceptor of electrons that have been produced from the splitting of water is
A)oxygen.
B)NADP+.
C)chlorophyll a.
D)carbon dioxide.
E)chlorophyll b.
9

All of the following are products of the light reactions of photosynthesis except
A)ATP.
B)oxygen.
C)NADPH.
D)glucose.
10

In oxygenic photosynthesis, water is split in order to provide the
A)O2 needed for the dark reactions.
B)electrons needed to reduce NADH.
C)electrons needed for cyclic photophosphorylation.
D)electrons needed to reduce P680.
11

The end products of noncyclic photophosphorylation are
A)O2, ATP, and NADPH.
B)carbon dioxide, PGAL, and H2.
C)water, ADP, and NADP+.
D)PGAL, ADP, and ribulose.
E)carbon dioxide, ATP, and water.
12

Carbon dioxide is reduced in
A)cyclic photophosphorylation.
B)noncyclic photophosphorylation.
C)the Calvin cycle.
D)the light reactions.
E)both light and dark reactions.
13

Which of the following is/are the products of the light reactions of photosynthesis?
A)ATP only
B)NADPH only
C)O2 only
D)ATP and O2 only
E)ATP, NADPH, and O2
14

All of the following produce oxygen as a product of photosynthesis except
A)cyanobacteria.
B)oak trees.
C)purple sulfur bacteria.
D)algae.
E)phytoplankton.
15

The chlorophyll molecules used by eucaryotes and cyanobacteria absorb radiant energy in the _____ portion(s) of the visible spectrum.
A)red
B)green
C)blue
D)green and ultraviolet
E)red and blue
16

The bacteriochlorophylls used by the anoxygenic bacteria have absorbance maxima located in the _____ portion(s) of the spectrum.
A)infrared
B)green
C)blue
D)ultraviolet
E)green and ultraviolet
17

Which of the following is an example of a polymer?
A)nucleotide
B)amino acid
C)monosaccharide
D)polysaccharide
E)fatty acid
18

Radioisotopes are frequently used in the study of cells. Assume a culture of E. coli is grown in a culture medium containing radioactive sulphur. At the end of 48 hours you would expect to find the radioactive label located in
A)enzymes.
B)DNA.
C)RNA.
D)phospholipids.
E)all of the above.
19

Radioisotopes are frequently used in the study of cells. Assume a culture of E. coli is grown in a culture medium containing radioactive phosphorous. At the end of 48 hours you would expect to find the radioactive label located in
A)enzymes.
B)DNA.
C)RNA.
D)phospholipids.
E)all of the above.
20

Which of the following is an example of an amphibolic pathway? <a onClick="window.open('/olcweb/cgi/pluginpop.cgi?it=jpg:: ::/sites/dl/free/0072320419/20545/ch10_num20_image.jpg','popWin', 'width=NaN,height=NaN,resizable,scrollbars');" href="#"><img valign="absmiddle" height="16" width="16" border="0" src="/olcweb/styles/shared/linkicons/image.gif"> (6.0K)</a>
A)See above image.
B)See above image.
C)See above image.
D)See above image.
E)None of the above, there is no such thing as an amphibolic pathway.

For the following set of questions use the following key to express the truth of statements I and II.



21

Anabolic and catabolic pathways do not get "mixed up" in the cytoplasmic milieu because some of the enzymes utilize different cofactors. Hence,

  1. NADP+ is usually used for catabolic pathways.
  2. NAD+ is usually used for anabolic pathways.
A)I only is true.
B)II only is true.
C)Both I and II are true.
D)Neither I nor II are true.
22

In order to get inorganic phosphorous into organic compounds, the phosphate ion is incorporated via

  1. substrate level phosphorylation.
  2. oxidative phosphorylation.
A)I only is true.
B)II only is true.
C)Both I and II are true.
D)Neither I nor II are true.
23

The major route for incorporation of ammonia (NH4+) into organic compounds is via

  1. reduction of pyruvate or alpha-ketoglutarate by enzymes.
  2. atmospheric nitrogen fixation.
A)I only is true.
B)II only is true.
C)Both I and II are true.
D)Neither I nor II are true.
24

Nitrogen fixation is a process that requires

  1. energy.
  2. an anaerobic environment.
A)I only is true.
B)II only is true.
C)Both I and II are true.
D)Neither I nor II are true.
25

During the carboxylation phase of the Calvin cycle, CO2 combines with
A)ribulose 1,5 - bisphosphate.
B)phosphoglyceraldehyde.
C)phosphoglyceric acid.
D)pyruvic acid.
E)oxaloacetic acid from the Krebs cycle.
26

During the reduction phase of the Calvin cycle, phosphoglyceric acid is reduced to _____ utilizing _____ as the reduction source.
A)phosphoglyceraldehyde; NADH+H+
B)ribulose 1,5 - bisphosphate; NADH+H+
C)phosphoglyceraldehyde; NADPH+H+
D)ribulose 1,5 - bisphosphate; NADPH+H+
E)pyruvic acid; NADPH+H+
27

Which of the following nucleoside diphosphates is used most often in carbohydrate anabolism?
A)Adenosine diphosphate
B)Guanine diphosphate
C)Thymine diphosphate
D)Cytosine diphosphate
E)Uridine diphosphate
28

Assimilatory sulfate reduction involves the nucleotide _____ during the incorporation of H2S in the production of _____.
A)ATP; methionine
B)UTP; methionine
C)ATP; cytosine
D)UTP; cytosine
E)GTP; cytosine
29

Incorporation of atmospheric N2 to NH4+ occurs via the process of
A)assimilatory nitrate reduction.
B)nitrogen fixation.
C)transamination.
D)deamination.
E)none of the above, atmospheric N2 cannot be incorporated into NH4+.
30

Which of the following describes how a purine nucleotide like adenosine or guanine triphosphate is synthesized?
A)the double ring structure of the purine base is synthesized first and then attached to the ribose sugar that has the three phosphates attached.
B)the single ring structure of the purine base is synthesized first and then attached to the ribose sugar that has the three phosphates attached.
C)the double ring structure of the purine base is constructed on top of ribose 5 - phosphate and is phosphorylated subsequently utilizing ATP.
D)the single ring structure of the purine base is constructed on top of ribose 5 - phosphate and is phosphorylated subsequently utilizing ATP.
E)None of the above describe the general strategy for synthesis of a purine nucleotide.
31

If radioactive bicarbonate was supplied to bacterial cells that were actively synthesizing fatty acids, you would expect to find the bulk of the radioactivity in
A)the fatty acids.
B)as cellular bicarbonate.
C)the cytoplasmic membrane.
D)nucleic acids.
E)none of the above places because bacteria cannot synthesize fatty acids.








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