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Microbiology, Fifth Edition
Microbiology, 5/e
Lansing M Prescott, Augustana College
Donald A Klein, Colorado State University
John P Harley, Eastern Kentucky University
Microbial Recombination and Plasmids

Self-Quiz

Choose the best answer



1

The transfer of genetic material between bacteria in direct physical contact is called
A)conjugation
B)transformation
C)transduction
2

The transfer of a naked fragment of DNA between bacteria is called
A)conjugation
B)transformation
C)transduction
3

The transport of bacterial DNA to other bacteria via bacteriaphages is called
A)conjugation
B)transformation
C)transduction
4

This type of recombination commonly occurs between a pair of homologous DNA sequences
A)general recombination
B)site-specific recombination
C)replicative recombination
5

This type of recombination does not require homologous sequences and is important for the integration of viral genomes into bacterial chromosomes
A)general recombination
B)site-specific recombination
C)replicative recombination
6

This type of recombination does not require homologous sequences and is utilized by mobile genetic elements that move about chromosomes
A)general recombination
B)site-specific recombination
C)replicative recombination
7

Horizontal transfer can best be described as:
A)the transmission of genetic information from parent to offspring
B)the transmission of genetic information from one independent, mature organism to another
C)the synthesis of RNA from a DNA template
8

Plasmids can best be described as:
A)small, circular DNA molecules that can exist independently of chromosomes commonly found in bacteria
B)a complex membrane structure that covers the chromosome of bacteria
C)another name for a chloroplast
9

The name of the process in which plasmids can be eliminated from a cell is:
A)fixing
B)breaking
C)curing
10

This type of plasmid can exist with or without being integrated into the host's chromosome
A)lysogen
B)episome
C)medisome
11

F factor plasmids play a major role in what bacterial process?
A)transduction
B)replication
C)conjugation
12

This type of plasmid makes the host more pathogenic
A)metabolic plasmid
B)virulence plasmid
C)F factors
13

This type of plasmid carries genes encoding enzymes that degrade substances such as aromatic compounds, pesticides or sugar
A)metabolic plasmid
B)virulence plasmid
C)F factors
14

Mobile genetic elements that carry the genes required for integration into host chromosomes
A)plasmids
B)transposons
C)replicon
15

This simplest transposable element contains inverted repeat sequences at each end and a gene encoding transposase.
A)composite transposon
B)insertion element
C)virus
16

The tranposase gene encodes an enzyme that:
A)facilitates general recombination
B)facilitates viral replication within a genome
C)facilitates site-specific integration of transposable elements.
17

Composite transposons are formed when
A)an IS element integrates into a plasmid
B)a small deletion occurs in the transposase gene of an IS element
C)two IS elements integrate into a chromosome with only a small distance between separating them
18

Which of the following are true in regards to F+ x F- mating events?
A)DNA is transferred from F+ to F- cells
B)DNA is transferred from F- to F+ cells
C)No DNA is transferred because F- cells are unable to perform conjugation
19

A bacterial cell that is able to take up naked DNA is said to be
A)competent
B)liable
C)infected
20

The relationship between a virus and host where no new viral particles are produces and the viral genome is replicated along with host chromosome
A)lysogeny
B)lysis
C)transformation
21

Interrupted mating experiments are used to determine what information?
A)DNA nucleotide sequences
B)levels of DNA homology
C)bacterial genome maps
22

Which of the following can be used as a tool by microbial genetisists?
A)plasmids
B)bacteriophage
C)transposable elements
D)all of the above
23

In which type of transduction does the transducing particle carry only specific portions of the bacterial genome?
A)general transduction
B)abortive transduction
C)specialized transduction
24

Recombination of virus genomes occurs
A)by transformation
B)by transduction
C)when two viruses with homologous chromosomes infect a host cell simultaneously
25

These are bacterial proteins that destroy other bacteria
A)viruses
B)transposable elements
C)bacteriocins
26

A replicon is
A)a DNA molecule that is able to replicate and be maintained
B)the enzyme responsible for transposition
C)a DNA molecule that encodes pili for conjugation








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