Multiple Choice Quiz


1	Which gene is incorporated into plasmids to detect recombinant cells?
		A)	restriction endonuclease
		B)	virus receptors
		C)	a gene for antibiotic resistance
		D)	reverse transcriptase


2	Which of the following is not essential to carry out the polymerase chain reaction?
		A)	primers
		B)	DNA polymerase
		C)	gel electrophoresis
		D)	high temperature


3	Which of the following must be present to make a nucleic acid hybrid?
		A)	Taq polymerase
		B)	a labeled probe
		C)	a plasmid
		D)	RNA


4	What do we call the synthetic unit of the polymerase chain reaction?
		A)	annealer
		B)	ligase
		C)	amplicon
		D)	primer


5	The function of ligase is to
		A)	rejoin segments of DNA
		B)	make longitudinal cuts in DNA
		C)	synthesize cDNA
		D)	break down ligaments


6	The pathogen of plant roots that is used as a cloning host is
		A)	Pseudomonas
		B)	Agrobacterium
		C)	Escherichia coli
		D)	Saccharomyces cerevisiae


7	Which of the following is a site that could be clipped by an endonuclease?
		A)	ATCGATCG, TAGCTAGC
		B)	AAGCTT, TTCGAA
		C)	ATATATA, TATATAT
		D)	ACCAT,TGGTA


8	The antisense DNA strand that complements mRNA AUGCGCGAC is
		A)	UACGCUCUG
		B)	GTCTCGCAT
		C)	TACGCTCTG
		D)	DNA cannot complement mRNA


9	Which DNA fragment will be closest to the top (negative pole) of an electrophoretic gel?
		A)	450 bp
		B)	3,560 bp
		C)	5 kb
		D)	1,500 bp


10	Which of the following is a primary participant in cloning an isolated gene?
		A)	restriction endonuclease
		B)	vector
		C)	host organism
		D)	all of these


11	For which of the following would a nucleic acid probe not be needed?
		A)	locating a gene on a chromosome
		B)	developing a Southern blot
		C)	identifying a microorganism
		D)	constructing a recombinant plasmid


12	Satellite DNA
		A)	contains important metabolic genes
		B)	is composed of highly repetitive sequences
		C)	is useless junk
		D)	causes cancer

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