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Multiple Choice Quiz
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1

The use or alteration of cells or biochemicals to provide a useful product describes
A)recombinant DNA technology.
B)transgenic technology.
C)biotechnology.
D)gene targeting.
2

Which of the technologies listed below is a valuable method for mass-producing drugs and other useful proteins?
A)recombinant DNA technology
B)transgenic technology
C)biotechnology
D)gene targeting
3

Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. Which of the tools below is used to cut the gene from its normal location?
A)restriction enzyme
B)plasmid
C)bacteriophage
D)vector
4

Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. Which of the below describe the circular piece of DNA from a bacterial cell?
A)restriction enzyme
B)plasmid
C)bacteriophage
D)vector
5

Which gene transfer technique involves a tiny needle which is used to inject DNA into a cell lacking that DNA sequence?
A)electroporation
B)liposome transfer
C)microinjection
D)particle bombardment
6

The process of ____ involves the introduction of a gene into a cell where it exchanges places with its counterpart in the host cell.
A)transgenic technology
B)gene targeting
C)knockout technology
D)recombinant DNA technology
7

A multicellular organism that carries a specific genetic change in each cell because of an intervention at the fertilized egg stage is a
A)transversion.
B)transition.
C)transgenic.
D)transformant.
8

The first drug produced using recombinant DNA technology is used to treat
A)hemophilia.
B)dwarfism.
C)heart attack.
D)diabetes.
9

Genetic engineering manipulates gene products at the level of the
A)protein.
B)amino acid.
C)DNA.
D)RNA.
10

A knockout mouse is so named because
A)it contains aggression genes.
B)it contains multiple copies of a human gene.
C)it contains an activated human gene.
D)none of the above.
11

The first patent for a transgenic organism was awarded in 1988. Which organism was patented?
A)a yeast used in industrial processes
B)a bacterium able to metabolize components of crude oil
C)a mouse that manufactures human protein in its milk
D)life forms cannot be patented
12

Palindromic sequences in DNA
A)form "blunt" ends when cut by restriction enzymes.
B)reflect the same sequence on two sides.
C)are not useful in recombinant DNA experiments.
D)all of the above.
13

A cDNA version of a gene includes
A)codons for a mature mRNA.
B)sequences corresponding to promoters.
C)sequences corresponding to introns.
D)both b and c.
14

Which of the following transgenic pharming products is incorrectly paired with the host organism that produces it?
A)hemoglobin - rabbit
B)human growth hormone - rat
C)lactoferrin - cows
D)alpha-1-antitrypsin - sheep
15

Swapping an inactivated allele for a gene of interest produces a ____ mouse while replacing a gene with another that has an altered function creates a ____ mouse.
A)knockout, knockout
B)knockout, gene targeted
C)knockout, knockin
D)gene targeted, knockin
16

Gene targeting is done on a(n)
A)sperm cell.
B)egg cell.
C)fertilized ovum.
D)early embryonic cell.
17

Human proteins can be produced in the milk or semen of farm animals. True or false?
A)true
B)false, proteins cannot be produced in milk
C)false, proteins cannot be produced in semen
D)false, animals are not used for protein production
18

____ consist of recombinant cells containing different fragments of a foreign genome.
A)DNA probes
B)Homologous recombinants
C)Genomic libraries
D)Knockout organisms
19

Recombinant DNA technology methods began in the
A)1950's.
B)1960's.
C)1970's.
D)1980's.
20

____ are used to select genes of interest from a genomic library.
A)Restriction enzymes
B)Cloning vectors
C)DNA probes
D)Gene targets
21

For the same gene, it is possible to patent
A)expressed sequence tags
B)cDNA
C)SNPs
D)all of the above
22

Which gene transfer technique involves the use of a fatty bubble to carry a gene into a somatic cell?
A)electroporation
B)liposome transfer
C)microinjection
D)particle bombardment
23

"Naked" DNA
A)is free of nucleic acids.
B)is free of the cell.
C)is free of protein.
D)contains just the sugar-phosphate backbone.
24

Which of the following gene-targeted mice have shown that a gene product once thought important is not vital to survival?
A)neurofibromatosis
B)X collagen
C)SCID
D)sickle-cell
25

The first drug produced using recombinant DNA technology was
A)streptokinase.
B)tPA.
C)insulin.
D)penicillin.







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