Review the animation and answer the questions that follow to test your understanding.

1			Synthesis of mRNA is
			A)	in the 5' to 3' direction with new nucleotides being added to the 5' end of the mRNA molecule.
			B)	in the 3' to 5' direction with new nucleotides being added to the 5' end of the mRNA molecule.
			C)	in the 5' to 3' direction with new nucleotides being added to the 3' end of the mRNA molecule.
			D)	in the 3' to 5' direction with new nucleotides being added to the 3' end of the mRNA molecule.
			E)	able to proceed either from the 5' to 3' or 3' to 5' direction.
2			Sigma factor is
			A)	a subunit of mRNA
			B)	a subunit of RNA polymerase
			C)	a subunit of DNA
			D)	an enzyme needed for transcription to proceed
			E)	the place on RNA where RNA polymerase attaches
3			Transcription begins when RNA polymerase binds to the
			A)	terminator on DNA
			B)	initiator on DNA
			C)	promoter on DNA
			D)	promoter on RNA
4			When RNA polymerase reaches a specific sequence of nucleotides on the DNA called the transcription terminator, a hairpin loop structure forms on the DNA strand causing the RNA polymerase and mRNA to dissociate from the DNA.
			A)	True
			B)	False
5			RNA polymerase copies the template strand of DNA making single-stranded mRNA complementary to the DNA template.
			A)	True
			B)	False