 | Chapter Objectives
(See related pages)
After reading this
chapter you should be able to: - discuss the use of recombinant DNA technology to genetically engineer various
organisms
- describe PCR and discuss its usefulness to genetic engineering and biotechnology
- discuss the key role played by restriction endonucleases and DNA ligase in
genetic engineering
- discuss how plasmids, phages, cosmids, and artificial chromosomes are used
as vectors for insertion and expression of foreign genes in an organism
- discuss the use of both procaryotes and eucaryotes as target organisms for
foreign gene insertion
- discuss the contributions already made by the use of this technology
- discuss the risks and the ethical problems associated with the use of this
technology
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