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Animation Quiz 1 - Sanger Sequencing
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Review the animation and answer the questions that follow to test your understanding.

1
The primer used in Sanger sequencing
A)can have any nucleotide sequence
B)must have a sequence beginning and ending with the same nucleotide
C)has a nucleotide sequence complementary to the 3' end of the region to be copied
D)has a nucleotide sequence complementary to the 5' end of the region to be copied
2
To carry out Sanger sequencing a mixture is needed containing
A)single-stranded DNA
B)DNA polymerase
C)four deoxyribonucleotides A, T, C, G
D)all of the above
3
When a dideoxyribonucleotide is added to the tube
A)replication of the strand continues
B)replication of the strand stops
C)replication of the strand is not affected
D)replication of the strand is speeded up
4
To sequence DNA by this method, the DNA must first be made in single stranded form.
A)True
B)False
5
Examining the electrophoresis gel and reading from bottom to top, one base at a time, gives the sequence of DNA.
A)True
B)False







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