 |
1 |  |  Thirty-six colonies grew in nutrient agar from 1.0 ml of sample withdrawn from a solution diluted to 10-5 in a standard plate count procedure. How many cells were in the original sample? |
|  | A) | 360 |
|  | B) | 3,600 |
|  | C) | 360,000 |
|  | D) | 1,800,000 |
|  | E) | 3,600,000 |
|
|
 |
2 |  |  Thirty-six colonies grew in nutrient agar from 0.1 ml of sample withdrawn from a solution diluted to 10-3 in a standard plate count procedure. How many cells were in the original sample? |
|  | A) | 360 |
|  | B) | 3,600 |
|  | C) | 360,000 |
|  | D) | 1,800,000 |
|  | E) | 3,600,000 |
|
|
 |
3 |  |  Which of the following is the best definition of generation time? |
|  | A) | The length of time it takes for lag phase. |
|  | B) | The length of time it takes for a population of cells to double. |
|  | C) | The maximum rate of doubling divided by the initial count. |
|  | D) | The duration of log phase. |
|  | E) | The time it takes for nuclear division. |
|
|
 |
4 |  |  An experiment began with 4 cells and ended with 128 cells. How many generations did the cells go through? |
|  | A) | 64 |
|  | B) | 32 |
|  | C) | 6 |
|  | D) | 5 |
|  | E) | 4 |
|
|
 |
5 |  |  If you start out with a population density of 200 CFU/ml of a bacterium that divides every 20 minutes, what will the population density be at the end of two hours, assuming the cells are in the log phase of growth. |
|  | A) | 1200 CFU/ml |
|  | B) | 26 CFU/ml |
|  | C) | 3200 CFU/ml |
|  | D) | 12800 CFU/ml |
|  | E) | 2006 CFU/ml |
|
|
Use this typical bacterial growth curve to answer the following question.
Compare the following entities (I and II).
 |
25 |  | 
I. average size of cells during the exponential phase of growth II. average size of cells during the lag phase of growth |
|  | A) | I is greater than II, mark A |
|  | B) | I is less than II, mark B |
|  | C) | I is exactly or approximately equal to II, mark C |
|  | D) | I may stand in more than one of the above relations to II, mark D |
|
|
 |
26 |  | 
I. generation time of cells during the exponential phase of growth II. generation time of cells during the lag phase of growth |
|  | A) | I is greater than II, mark A |
|  | B) | I is less than II, mark B |
|  | C) | I is exactly or approximately equal to II, mark C |
|  | D) | I may stand in more than one of the above relations to II, mark D |
|
|
 |
27 |  | 
I. generation time of a culture that produces 2 generations per hour II. generation time of a culture that produces 3 generations per hour |
|  | A) | I is greater than II, mark A |
|  | B) | I is less than II, mark B |
|  | C) | I is exactly or approximately equal to II, mark C |
|  | D) | I may stand in more than one of the above relations to II, mark D |
|
|
 |
28 |  | 
I. number of viable cells detected in a culture using an electronic counting devise II. number of viable cells detected in the same culture using a dilution and spread plating procedures |
|  | A) | I is greater than II, mark A |
|  | B) | I is less than II, mark B |
|  | C) | I is exactly or approximately equal to II, mark C |
|  | D) | I may stand in more than one of the above relations to II, mark D |
|
|
 |
29 |  |  The total biomass of an organism will be determined by the nutrient present in the lowest concentration relative to the organism's requirements is a statement of |
|  | A) | Liebig's Law of the minimum. |
|  | B) | Shelford's law of tolerance. |
|  | C) | the second law of thermodynamics. |
|  | D) | quorum sensing. |
|  | E) | Heisenberg's principle of uncertainty. |
|
|
 |
30 |  |  The ability of Vibrio fischeri to produce bioluminescence chemicals only when a certain population density has been reached is an example of |
|  | A) | Liebig's Law of the minimum. |
|  | B) | Shelford's law of tolerance. |
|  | C) | the second law of thermodynamics. |
|  | D) | quorum sensing. |
|  | E) | Heisenberg's principle of uncertainty. |
|
|