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Molecular Biology, 4/e
Robert F. Weaver

DNA Replication I: Basic Mechanism and Enzymology

DNA Replication Fork

View the animation below, then complete the quiz to test your knowledge of the concept.



1

The enzyme that unwinds a segment of the DNA molecule is...
A)DNA polymerase.
B)DNA ligase.
C)RNA primase.
D)DNA polymerase III.
E)helicase.
2

The enzyme that travels along the leading strand assembling new nucleotides on a growing new strand of DNA is...
A)DNA polymerase.
B)DNA ligase.
C)RNA primase.
D)DNA polymerase III.
E)helicase.
3

Before the lagging strand can begin assembling new DNA nucleotides, which of the following must occur?
A)DNA polymerase III adds deoxyribonucleotides.
B)DNA polymerase I removes some material and replaces it with DNA.
C)OH bonds must be broken between the two strands of DNA.
D)DNA ligase forms a phosphodiester bond between the 3' OH of the growing strand and the 5' phosphate in front of it.
E)RNA primase constructs a short RNA primer.
4

During DNA replication, the lagging strand is synthesized continuously, while the leading strand is synthesized discontinuously.
A)True
B)False
5

Before DNA replication can occur, the OH bonds between the strands must be broken.
A)True
B)False