Student Center
|
Instructor Center
|
Information Center
|
Home
Web Links
Lecture PowerPoints
Image PowerPoints
Choose a Chapter
Chapter 1
Chapter 2
Chapter 3
Chapter 4
Chapter 5
Chapter 6
Chapter 7
Chapter 8
Chapter 9
Chapter 10
Chapter 11
Chapter 12
Chapter 13
Chapter 14
Chapter 15
Chapter 16
Chapter 17
Chapter 18
Chapter 19
Chapter 20
Chapter 21
Chapter 22
Chapter 23
Chapter 24
DNA Probe (DNA Hybridization)
Electrophoresis
FISH
Southern Blot
Feedback
Help Center
Molecular Biology, 4/e
Robert F. Weaver
Molecular Tools for Studying Genes and Gene Activity
Electrophoresis
How does electrophoresis separate molecules?
Electrophoresis is a versatile tool in molecular biology. There are many different types of electrophoresis but they all work on the same principle. Samples are placed on one end of a ‘gel’. The gel is a substance that consists of a molecular matrix of evenly sized and spaced openings. An electrical current is passed through the gel. The current moves the molecules in the sample through the gel. The rate at which the molecules move depends on both their size and their charge. Large molecules will be slowed by the matrix while small molecules will move more quickly. Molecules with a charge will move rapidly while uncharged molecules will move slowly.
View the animation below, then complete the quiz to test your knowledge of the concept.
1
The first phase of 2-D gel electrophoresis separates proteins based on their_______.
A)
mass
B)
length
C)
isoelectric point
D)
density
E)
none of the above
2
The second phase of 2-D gel electrophoresis separates proteins based on their_______.
A)
mass
B)
length
C)
isoelectric point
D)
density
3
At the end of a 2-D gel electrophoresis, proteins with a high mass would be found where?
A)
along the left edge of the gel
B)
along the right edge of the gel
C)
along the top edge of the gel
D)
along the bottom edge of the gel
4
In the tube gel, each band represents a single type of protein.
A)
True
B)
False
5
2-D gel electrophoresis may be used to isolate specific proteins for purification and additional study.
A)
True
B)
False
2008 McGraw-Hill Higher Education
Any use is subject to the
Terms of Use
and
Privacy Policy
.
McGraw-Hill Higher Education
is one of the many fine businesses of
The McGraw-Hill Companies
.