1
Nucleosomes inhibitA) activators B) RNA polymerase C) translation D) assembly of transcription factors 2
Unlike prokaryotes, the control of transcription by eukaryotes is designed to react to change byA) changing B) ignoring change C) remaining constant D) changing the environment 3
A form of binding motif containing a nearly identical sequence of 60 amino acids in many eukaryotes is theA) homeodomain motif B) zinc finger motif C) leucine zipper motif D) universal motif 4
Which of the following does not occur in the function of the catabolite activator protein (CAP) of E. coli?A) Cyclic-AMP binds to the CAP protein. B) The protein changes shape. C) Space is increased by the binding of tryptophan. D) Helix-turn-helix motifs are enabled to bind to the DNA. 5
All of the following can be found in a human transcription complex exceptA) activator B) RNA C) enhancer D) silencer 6
Enhancers areA) proteins located adjacent to promoters B) distant sites where regulatory proteins bind C) expediters of RNA polymerase capture D) proteins that bind with repressors, deactivating them E) a bacterial form of promoters 7
When tryptophan is present in the medium, the transcription of tryptophan producing genes in E. coli is stopped by a helix-turn-helix regulator binding to theA) trp repressor B) trp operon C) trp promoter D) trp operator E) trp polymerase 8
When a homeodomain binds to DNA, the actual binding portion of the homeodomain isA) a leucine zipper B) the operon C) zinc fingers D) the histine E) a helix-turn-helix motif 9
The assembly of transcription factors on a promoter begins some 25 nucleotides upstream where it binds to a start _______________ sequence.A) ATAT B) AATT C) TTAA D) TAAT E) TATA 10
When tryptophan is present in the environment of E. coli, the tryptophan binds to theA) trp operon B) trp promoter C) trp operator D) trp repressor E) trp polymerase 11
Transcription factors appear to be unable to bind to a nucleosome becauseA) activators are inhibited by the configuration B) of inhibition of RNA polymerase C) of histones positioned over promoters D) nucleosomes are especially vulnerable to repressors E) operators are placed in an inaccessible position 12
In the zinc fingers motif, the spacing of the helical segments is performed byA) beta sheets B) helical clusters C) zinc atoms D) gamma helices E) an alpha helix 13
Translation repressor proteins may shut down translation of processed mRNA transcripts byA) binding with the poly-A tail B) resetting the reading frame C) reinserting introns into the transcript D) excising a short sequence of nucleotides 14
In many animals, the genes that regulate the development of stem cells are activatedA) once B) only twice C) up to 10 times D) over a hundred times E) not at all 15
The leucine zipper motif involves the cooperation of two _______________ subunits.A) leucine B) protein C) RNA D) polymerase E) histone 16
Regulatory domains of most activators interact withA) the transcription factor complex B) RNA polymerase C) repressors D) the regulatory factor complex E) the DNA binding domain 17
The operon that controls tryptophan producing genes in E. coli consists of _______________ .A) only one gene B) two genes C) three genes D) four genes E) five genes 18
In order for a gene to be transcribed, RNA polymerase must have access to the DNA helix and be able to bind to the genesA) activator B) regulator C) promoter D) operator E) repressor 19
In the function of the lac operon in E. coli, the lac genes are transcribed in the presence of lactose becauseA) RNA polymerase binds to the operator B) the repressor cannot bind to the promoter C) an isomer of lactose binds to the repressor D) CAP does not bind to the operator E) of the absence of cAMP 20
The role of methylation of DNA is now viewed asA) interfering with DNA transcription by blocking base pairing between cytosine and guanine B) complexing with enhancers to prevent transcription C) prevention of mutation D) insuring that genes that are turned off, stay off E) irrelevant to gene transcription 21
In order for the helix-turn-helix motif to bind to DNA, the _______________ must fit into the major groove of the DNA.A) homeotic switches B) zinc fingers C) operator D) recognition helix E) protein link 22
The most common form of gene expression regulation in both bacteria and eukaryotes isA) translational control B) transcriptional control C) post-transcriptional control D) post-translational control E) control of passage from the nucleus 23
In eukaryotes, many genes may have to interact with each other, requiring more interacting elements than can fit around a single promoter. This physical limitation is overcome byA) alternating promoters and operators B) placing promoters on both sides of each gene C) the use of very long promoters D) distant sites in a chromosome controlling transcription of a gene E) having factors on one chromosome control genes on another gene 24
E. coli is able to use foods other than glucose in the absence of available glucose, because falling levels of glucose cause an increase ofA) cAMP B) CAP C) lactase D) glu operons E) tRNA 25
In the absence of glucose, E. coli can import lactose to change into glucose and galactose because CAP binds to theA) cAMP B) DNA C) lac operon D) operator E) repressor 26
Which is not part of the lac operon?A) repressor B) activator protein C) operator D) promotor E) structural gene 27
In an operon the location of the regulatory region occurs ________ the structural genes.A) after B) within C) before 28
In eukaryotic gene regulation, the location of the promoter is always before and the location of the enhancer always after the gene being regulated.A) True B) False 29
Proteins that block the passage of RNA polymerase are called:A) operons B) activators C) repressors D) enhancers E) promoters 30
Which of the following is part of an operon?A) structural genes B) an operator C) a promoter D) a CAP binding site E) all of the above 31
Which of the following are not matched correctly?A) exon splicing-occurs in nucleus B) post-translational modifications-phosphorylation C) snRNA-splice out exons from transcript D) activated enhancers-trigger transcription E) all are matched correctly 32
A single gene may use a regulatory site to control the expression of that gene, but genes rarely have multiple regulatory sites.A) True B) False