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Biology, 6/e
Author Dr. George B. Johnson, Washington University
Author Dr. Peter H. Raven, Missouri Botanical Gardens & Washington University
Contributor Dr. Susan Singer, Carleton College
Contributor Dr. Jonathan Losos, Washington University

Gene Technology

Multiple Choice Quiz

Please answer all questions



1

Clones are identified by hybridizing them with
A)a vector
B)an antibody
C)a virus
D)a probe
2

The first step in the polymerase chain reaction (PCR) is
A)denaturation
B)primer extension
C)annealing
D)cooling
3

In genome sequencing, it was found that ____ % of the Drosophila genes matched other species.
A)45
B)72
C)98
D)83
4

With the completion of the human genome project, the next frontier is
A)nucleonics
B)proteomics
C)cytomics
D)agrinomics
5

By using the "reference sequence" developed in the human genome project, individual differences are now being detected using
A)NPs
B)PSNs
C)SNPs
D)"snaps"
6

In the discovery of introns, a DNA molecule called _______________ was formed that had the same nucleotide sequence as the gene that produced the mRNA.
A)mDNA
B)rDNA
C)sDNA
D)gDNA
E)cDNA
7

Viruses containing RNA rather than DNA are called
A)riboviruses
B)immunoviruses
C)bacteriophages
D)rotaviruses
E)retroviruses
8

In a polymerase chain reaction, a synthetic sequence of nucleotides are involved in
A)denaturing
B)heating
C)priming
D)copying
E)all of the above
9

Bacteria protect themselves from viruses by fragmenting viral DNA upon entry with
A)ligases
B)endonucleases
C)methylases
D)vectors
E)probes
10

In preliminary screening of clones, it is common to use
A)restriction enzymes
B)dyes
C)antibiotics
D)radiation
E)milipore filters
11

To identify an individual by DNA analysis of their blood, investigators look for
A)primers
B)DNA fingerprints
C)probes
D)nucleosomes
E)transgenic fragments
12

All fragments cut by most restriction endonucleases have
A)complementary double-stranded ends
B)supplementary single-stranded ends
C)double-stranded "sticky" ends
D)complementary single-stranded ends
E)double-stranded supplementary ends
13

In 1980, interferon was produced by splicing a human gene into the genome of
A)bacteria
B)yeast
C)viruses
D)mice
E)rabbits
14

The second step in most genetic engineering experiments is
A)screening
B)production of recombinant DNA
C)cleavage of DNA
D)cloning
E)testing
15

When "sticky ends" are paired, they can be joined by
A)restriction enzymes
B)pSC101
C)methylase
D)X-gal
E)DNA ligase
16

A successful vector in genetic engineering has been the
A)TMV plasmid
B)HLF virus
C)vaccinia virus
D)Ti plasmid
E)retrovirus
17

Humans are not affected by glyphosphate in plant crops because
A)while it is toxic to plants it is a normal metabolite of humans
B)humans do not produce aromatic amino acids
C)it is used only on those crops that convert it to a harmless substance
D)humans lack the enzymes to interact with it
E)humans have EPSP synthetase
18

In the screening process, clones that metabolize X-gal turn
A)yellow
B)orange
C)red
D)blue
E)colorless
19

A powerful way to identify an individual using a particular gene as a marker is the analysis of
A)RFLP's
B)X-gal reaction
C)PCR's
D)EcoRI's
E)BST's
20

In attempts to confer special characteristics upon plants, genetic engineers find Agrobacterium tumefaciens to be an effective vector for use with
A)corn
B)rice
C)wheat
D)soy beans
E)barley
21

A library of DNA fragments results from the use of
A)restriction endonucleases
B)virus
C)plasmids
D)recombinant DNA
E)DNA ligase
22

One of the most useful methods for identifying a specific gene is
A)thin layer chromatography
B)the Eastern blot
C)the Western blot
D)magnetic resonance imaging
E)the Southern blot
23

Plant cells receiving Ti plasmids with a TMV viral gene encoding the viral coat protein become immune to the TMV virus because
A)the introduction of the viral gene triggered an immune response in the plant's own immune system
B)the gene causes the cell walls to become impermeable to the virus
C)TMV does not infect cells already infected with TMV
D)in plants, the gene encodes an antiviral substance
E)none of the above
24

Bacterial DNA is not cleaved by their own restriction enzymes because bacteria add _______________ to their own DNA.
A)nucleotides
B)peptides
C)methyl groups
D)glyphosphate
E)somototropin
25

A procedure called PCR is used to
A)cleave DNA
B)produce recombinant DNA
C)copy gene sequences
D)clone cells
E)screen clones
26

Wilt-proof flowers are being engineered by making them insensitive to
A)methylene
B)propylene
C)butylene
D)acetylene
E)ethylene
27

The ingestion of BST in milk is no threat to humans because
A)it is destroyed in the stomach
B)it cannot be digested and will pass through as waste
C)they do not have the hormones to utilize it
D)it is destroyed in the pasteurization process
E)the FDA has ruled that such milk cannot be sold
28

Genetic engineering has successfully transferred genes from eukaryotic cells into:
A)bacteria
B)plants
C)animals
D)all of the above
E)none of the above
29

DNA molecules can be cut into sections by using:
A)ATP
B)gel electrophoresis
C)restriction endonucleases
D)plasmids
E)a probe
30

In genetic engineering, DNA ligase is used as:
A)a probe
B)a sealing enzyme
C)a restriction enzyme
D)a mutagen
E)none of the above
31

In Cohen and Boyer's transfer of toad genes to a bacterial cell, restriction endonucleases were used to:
A)isolate sections of cloned bacterial plasmids
B)isolate sections of toad DNA
C)cleave bacterial plasmids
D)Both a and b are correct.
E)Both b and c are correct.
32

A probe is used in which stage of the gene transfer process?
A)cleaving DNA
B)recombining DNA
C)cloning
D)screening
E)both a and b
33

The Polymerase Chain Reaction is used to:
A)amplify a small amount of DNA
B)cleave bacterial plasmids
C)seal "sticky ends"
D)identify target plasmids
E)none of the above
34

Which of the following statements is true about developing cDNA?
A)mature mRNA directs the formation of the DNA.
B)mature mRNA does not contain introns.
C)DNA taken from the nucleus is used to produce the cDNA.
D)Both a and b are true.
E)none of the above are true.
35

Genetic engineering has been used to do all of the following except:
A)make plants more resistant to frost
B)make plants more resistant to disease
C)make plants more resistant to herbicides
D)improve the nutritional balance of plants
E)all of the above are correct.
36

Engineering plants that are resistant to glyphosate was an important advancement because:
A)glyphosate promotes frost damage
B)glyphosate encourages the production of fruit that is lower in protein
C)glyphosate is the active ingredient in herbicide
D)glyphosate prevents the transfer of genes into the plants
E)none of the above
37

The use of bovine growth hormone:
A)has raised controversy with groups opposed to genetic engineering
B)increased milk production in cows
C)is being tested on increasing the weight gain of cows
D)is being tested as a treatment for dwarfism in humans
E)all of the above
38

The Ti plasmid provides a means to transfer genes into cereal plants, such as corn and rice.
A)True
B)False
39

Genetic engineering has provided a method for the production of insulin to fight diabetes, but it remains too expensive to be practical.
A)True
B)False
40

Piggyback vaccines work by triggering the body's defense system with a harmless virus carrying the surface proteins of a disease organism.
A)True
B)False
41

The second stage in genetic engineering experiments is cloning the plasmid.
A)True
B)False
42

When using reverse transcriptase, DNA is made from mRNA.
A)True
B)False
43

Genetically identical organisms derived from a single genetic source are called
A)populations
B)varieties
C)sibling species
D)ecotypes
E)clones