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| 1 | If you diligently wash hands before and after lab, you don’t need to wear gloves. |
 | A) | True |
| B) | False |
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| 2 | Safety considerations in the Microbiology laboratory are crucial for protecting personnel. However, it is also important to think about “protecting” the microorganisms in the experiment that you are performing and not contaminating your culture. Lab workers who are carriers, or who are sick, can contaminate cultures. A pure culture could also be contaminated with another lab strain. What precautions must be taken to prevent contamination of a pure culture? |
| A) | Use of gloves, disinfecting gloves between handling cultures, handling cultures only in a biosafety cabinet, and using lab materials for only 2-3 cultures prior to disposal or autoclaving |
| B) | Use of gloves, disinfecting gloves between handling cultures, handling cultures on an open bench top, use lab materials for only one culture before disposal or autoclaving |
| C) | Use of gloves, disinfecting gloves between handling cultures, handling cultures only in a biosafety cabinet, use lab materials for only one culture before disposal or autoclaving |
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| 3 | In the following scenario, where could laboratory transmission occur? : A microbiology student was performing a lab exercise. While performing this exercise the student was wearing a lab coat, eye goggles, and gloves. As the exercise was progressing the student diligently recorded the data with a pen, still wearing gloves. At the end of the lab, the student removed her lab coat, goggles and gloves. She proceeded to wash her hands and place her pen into her backpack. |
| A) | students clothes under the lab coat |
| B) | lack of hand washing |
| C) | students pen |
| D) | students backpack |
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| 4 | As stated in the lab manual most microbiology laboratories are designed to handle BSL-1 and BSL-2 rated organisms. If you were given an unknown culture in the lab, which biosafety level would you observe? |
| A) | BSL-1, always start at the lowest level and increase the level/precautions as data presents itself |
| B) | BSL-2, or the highest level available in your laboratory |
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| 5 | Beginning with BSL-2 and beyond, which type of equipment must be accessible for decontamination of biohazardous waste generated in the lab? |
| A) | Refrigerator |
| B) | Incubator |
| C) | Sink for handwashing |
| D) | Safety shower |
| E) | Autoclave |