Post Quiz (See related pages) 1 Cells that are not acid-fast are easily decolorized using the reagent _________. A) ethyl alcohol B) acid alcohol C) water D) Gram's iodine E) heat 2 Acid-fast staining was performed on a sputum sample from a patient exhibiting clinical signs of Tuberculosis, red cells were observed on the slide. What can be determined from this slide? A) The sample contained endospore forming bacteria B) The sample contained non-acid-fast cells, suggestive of members of the genus Mycobacterium C) The sample contained bacteria with mycolic acid in their cell walls, suggestive of members of the genus Mycobacterium 3 What color will the colonies of lactose fermenting bacteria appear when grown on MacConkey agar? A) Red B) Pink C) White D) Colorless 4 A Case Study. The sputum from two patients was being tested for the presence of M. tuberculosis . Patient 1exhibited clinically obvious TB. Patient 2 did not exhibit clinically obvious TB, but had been in contact with patient 1. The process of testing the sputum samples from patients 1 and 2 included the use of a positive control (test sample/culture). M. tuberculosis was isolated from the sputum samples of both patients. DNA fingerprinting revealed that the isolates from patients 1 and 2 were not identical. Which of the following possibilities most likely occurred? A) Both patients were infected from a common source B) Laboratory error resulted in one patient specimen being contaminated with another patient specimen C) Poor handling of the test culture allowed cross contamination to patient 2 5 In the M. tuberculosis case described in this exercise, which of the following situations is a way in which cross-contamination could have occurred in the medical laboratory? A) Flaming the necks of tubes containing cultures B) Sterilizing inoculating tools in the flame of a Bunsen burner C) Using hot inoculating tools D) Using the lid of a petri dish as a shield
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