PreQuiz (See related pages) 1 Once milk has gone through the process of pasteurization it is considered sterile. A) True B) False 2 Raw milk is a source of what kind of outbreaks? A) Salmonella and Escherichia coli B) Staphylococcus aureus and Streptococcus pyogenes C) Pseudomonas aeruginosa and Enteroccus faecalis 3 Two microbiology students performed the viable plate count exercise, as described in this lab manual. Both students performed the procedure using the same sample of pasteurized milk, however their final coliform and total bacterial colony counts varied greatly. Which of the following scenarios could have caused this variation? A) Improper dilutions B) Not mixing the milk before dilutions C) Nonsterile water used for dilutions D) Spreading the inoculum with a sterile spreader that had not cooled E) Immediately inverting the inoculated plates F) All of the above 4 MacConkey Agar selects for growth of which type of organism? A) Gram-positive B) Gram-negative 5 A plate inoculated with a 1:100 dilution of milk produced 30 colonies. How many organisms are in 1 ml of the undiluted sample? A) 30 B) 300 C) 3000 D) 30000
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