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1Keeping in mind that certain disinfectants evaporate faster than others, which of the following disinfectants should not be used in a procedure that requires 30 minutes of contact time between a bench top and the disinfectant?
A)Sodium hypochlorite
B)Hydrogen peroxide
C)Amphyl/Lysol
D)Ethanol


2An undiluted disinfectant is always more effective than a diluted disinfectant.
A)True
B)False


3Lysol works by disrupting bacterial proteins and the lipid membrane, it has a broad spectrum of action. Triclosan is a disinfectant that works by acting on a single specific protein that bacteria require to make an effective lipid membrane. Triclosan has a specific target and does not have a broad spectrum of action. Is a microorganism more likely to develop resistance to a disinfectant that has a broad spectrum of action such as lysol, or a disinfectant that has a specific target in a bacterial cell such as Triclosan?
A)A disinfectant that has a broad spectrum of action.
B)A disinfectant that has a specific target in a bacterial cell.


4If a culture of Bacillus anthracis containing endospores was used in this experiment, do you think this culture would be more or less susceptible to the chemical disinfectants than Pseudomonas aeruginosa?
A) Bacillus anthracis would be more susceptible to chemical disinfection than Pseudomonas aeruginosa
B) Bacillus anthracis would be less susceptible to chemical disinfection than Pseudomonas aeruginosa


5For surfaces that have not been cleaned for a long time, sometimes applying a proven disinfectant does not work. These surfaces also must be scrubbed (agitated). Why does scrubbing the surface make the disinfectant work better?
A)Bacteria may have formed a biofilm, and scrubbing the surface helps the disinfectant penetrate the slime layer of the biofilm.
B)The scrubbing action oxidizes the disinfectant, and improves its efficacy.
C)Scrubbing often produces bubbles, the bubbles improve the efficacy of the disinfectant.





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