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1Imagine that a random mutation results in a bacterial cell gaining the ability to grow in an environment with a salt concentration of 13%. The population this mutated cell arose from is growing in an environment with a salt concentration of 1.0%. Over the next 3 weeks the salt concentration of the environment fluctuates between 0.9% and 1.0%. What would you predict might happen to the population over these three weeks?
A)In this example the ability to grow in an increased salt concentration provided the mutated cell with a distinct advantage, therefore the mutated cell was selected for and it began to dominate over the three week period.
B)In this example the ability to grow in an increased salt concentration did not provide the mutated cell with any advantage, it was not selected for and did not dominate the population over the three week period.


2In this experiment, what happens when arabinose is present in the environment of cells transformed with pGLO?
A)When arabinose is present in the environment RNA polymerase is not able to transcribe the genes in the operon.
B)When arabinose is present in the environment RNA polymerase is able to transcribe the genes in the operon, producing the enzymes needed to digest arabinose.


3In this experiment the pGLO plasmid is used. Is the arabinose promoter of this plasmid a constitutive or an inducible promoter?
A)Constitutive
B)Inducible


4In this experiment if the bacterial colonies do not fluoresce under ultraviolet light, what can you determine about the GFP gene?
A)If there is no fluorescence under ultraviolet light the GFP gene is being transcribed
B)If there is no fluorescence under ultraviolet light the GFP gene is not being transcribed


5Which of the following genes of the pGLO plasmid allow it to grow in the presence of the selective agent used in this lab?
A)bla gene
B)GFP gene
C)araC gene





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