Post Quiz (See related pages) 1 What molecule must be added to the media for a strain of bacteria auxotrophic for alanine to grow? A) Alanine B) Tryptophan C) Hisitidine D) Biotin 2 Observe Figure 25.2(a) of your lab manual. The disc in the center of the plate is saturated with a chemical being tested for mutagenic properties. As you know, this chemical has mutagenic properties because there are numerous revertant colonies on the plate. The concentration of the test chemical is highest in areas closest to the disc, and lowest in areas furthest from the disc. Why aren’t there revertant colonies in areas where the test chemical (which causes the reversions) is the greatest? A) In high concentrations the test chemical is toxic B) In high concentrations the test chemical causes DNA repair C) In high concentration the test chemical causes spontaneous mutations 3 The Ames test measures the ability of a chemical to revert a prototroph back to its original auxotrophic state. A) True B) False 4 Why is it important to select a test compound that does not contain protein? A) Protein in the test compound may cause back mutations B) Protein in the test compound may inhibit the growth of Salmonella typhimurium C) Protein in the test compound may provide the Salmonella typhimurium TA 1538 with the histidine it needs to grow on His- plates 5 After incubation, Salmonella typhimurium TA 1538 colonies found on the control plate with the filter paper disk saturated with water (the negative control) are _________. A) auxotrophic for histidine B) prototrophs
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