Post Quiz (See related pages) 1 Gram staining, capsular staining, and phage typing are tests used to help identify bacteria. These tests are based on _______ differences in bacteria. A) phenotypic B) genotypic 2 In this experiment, step three uses a protease along with heat to denature and digest proteins within the cell. Does the protease or the heat digest the protein? A) Protease digests proteins and heat denatures proteins B) Heat digest proteins and protease denatures proteins 3 In this experiment an ice bath is used and ice cold isopropyl alcohol. If this procedure is performed and care is not taken to use ice cold alcohol, the yield of DNA decreases. Why do you think it is important to use ice cold alcohol? A) Cold temperatures slow down enzymes, thus protecting DNA from enzymes that could break it down. B) Cold temperatures help DNA to precipitate and not become soluble. C) Both of the choices above help explain why ice cold alcohol should be used. 4 Often students are surprised after performing this exercise at the fact that the bacterial pellet was quite small, and yet they were able to see the DNA without the aid of magnification. Which of the following choices provides a good explanation as to why the amount of DNA may seem large? A) In this procedure the DNA is replicated B) While in cells DNA is extremely compact within the double helix (supercoiled), the DNA isolated in this protocol is no longer compact C) DNA swells after it has been extracted from bacterial cells 5 In analyzing an outbreak of disease, the first thing is to identify the bacterial species involved. Then once you know the species you can move forward to determine if the outbreaks were all caused by the same strain. Which of the following tests can be used to help determine the species involved? A) Phage typing B) PCR C) DNA fingerprinting D) Gram-staining
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