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1Although invisible to the naked eye, what kind of reaction occurs in a capture ELISA when complimentary test antigen is added to wells coated with antibodies?
A)Synthesis reaction
B)Agglutination reaction
C)Dissociation


2Which part of the structure of an antibody differs from antibody to antibody, allowing each antibody to recognize a different antigen.
A)The constant region of the heavy chain
B)The constant region of the light chains
C)The disulfide bonds
D)The variable region at the upper tips of the Y of an antibody


3A capture ELISA is performed on serum from a patient with an ongoing infection. The first serum sample was taken 1 week after exposure to the antigen, while the second serum sample was 3 weeks post-exposure. Wells containing serum from the first sample turned bright yellow at the end of the ELISA, while wells containing serum from a later time point turned pale yellow. Which of the following conclusions can be drawn from this data?
A)One week post-exposure there is less antigen than three weeks post exposure.
B)One week post-exposure there is more antigen than three weeks post exposure.


4An indirect ELISA was performed on the serum samples described in question 3. The two samples used in the ELISA were taken from a patient one week post exposure to antigen and three weeks post exposure to antigen. The indirect ELISA measured the levels of antibody specific to the antigen. Based on what you know about antibody generation and the answer to question 3, what would you expect the results of the indirect ELISA to look like?
A)Wells containing serum one week post exposure would be light yellow, while wells containing serum 3 weeks post exposure would be dark yellow.
B)Wells containing serum one week post exposure would be dark yellow, while wells containing serum 3 weeks post exposure would be light yellow.


5In an indirect ELISA the secondary antibody is specific for the known antigen.
A)True
B)False





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