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1Which of the following cellular stains is considered a differential stain?
A)Methylene blue
B)Crystal violet
C)Gram stain
D)India ink



2The Gram stain separates bacteria based on _________.
A)the charge of the cell (either positive or negative)
B)the presence or absence of nuclei
C)differences in the structure of the cell wall



3If a student wants to accurately determine the size of bacterial cells in a culture, which of the following dyes would you recommend they use?
A)Methylene blue
B)Crystal violet
C)Safranin
D)India ink



4Which of the following chemicals acts as a mordant in the Gram stain procedure?
A)Crystal violet
B)Iodine
C)Ethyl alcohol
D)Safranin



5Gram-positive cultures older than 24 hours may stain Gram negative due to changes in the peptidoglycan layer.
A)True
B)False







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