About the Author

Preface

Acknowledgments

Guide to Experimental Techniques in Molecular Biology

PART I Introduction

Chapter 1

A Brief History

Chapter 2

The Molecular Nature of Genes

Chapter 3

An Introduction to Gene Function

PART II Methods in Molecular Biology

Chapter 4

Molecular Cloning Methods

Chapter 5

Molecular Tools for Studying Genes and Gene Activity

PART III Transcription in Bacteria

Chapter 6

The Mechanism of Transcription in Bacteria

Chapter 7

Operons: Fine Control of Bacterial Transcription

Chapter 8

Major Shifts in Bacterial Transcription

Chapter 9

DNA–Protein Interactions in Bacteria

PART IV Transcription in Eukaryotes

Chapter 10

Eukaryotic RNA Polymerases and Their Promoters

Chapter 11

General Transcription Factors in Eukaryotes

Chapter 12

Transcription Activators in Eukaryotes

Chapter 13

Chromatin Structure and Its Effects on Transcription

PART V Posttranscriptional Events

Chapter 14

RNA Processing I: Splicing

Chapter 15

RNA Processing II: Capping and Polyadenylation

Chapter 16

Other Post-Transcriptional Events

PART VI Translation

Chapter 17

The Mechanism of Translation I: Initiation

Chapter 18

The Mechanism of Translation II: Elongation and Termination

Chapter 19

Ribosomes and Transfer RNA

PART VII DNA Replication, Recombination, and Transposition

Chapter 20

DNA Replication, Damage, and Repair

Chapter 21

DNA Replication II: Detailed Mechanism

Chapter 22

Homologous Recombination

Chapter 23

Transposition

PART VIII Genomes

Chapter 24

Introduction to Genomics: DNA Sequencing on a Genomic Scale

Chapter 25

Genomics II: Functional Genomics, Proteomics, and Bioinformatics

Glossary

Index