 |  Microbiology, 5/e Lansing M Prescott,
Augustana College
Donald A Klein,
Colorado State University
John P Harley,
Eastern Kentucky University
The Study of Microbial Structure: Microscopy and Specimen Preparation
Chapter ObjectivesAfter reading this
chapter you should be able to:
- describe how lenses bend light rays to produce enlarged images of small objects
- describe the various parts of the light microscope and how each part contributes to the functioning of the microscope
- describe the preparation and simple staining of specimens for observation with the light microscope
- describe the Gram-staining procedure and how it is used to categorize bacteria
- describe the basis for the various staining procedures used to visualize specific structures associated with microorganisms
- compare the operation of the transmission and scanning electron microscopes with each other and with light microscopes
- describe confocal microscopy and scanning probe microscopy
- compare and contrast light microscopes, electron microscopes, confocal microscopes, and scanning probe microscopes in terms of their resolution, the types of specimens that can be examined, and the images produced
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2002 McGraw-Hill Higher Education