Microbiology, 5/e

Lansing M Prescott, Augustana College
Donald A Klein, Colorado State University
John P Harley, Eastern Kentucky University

The Study of Microbial Structure: Microscopy and Specimen Preparation

True or False Quiz


1	In a compound microscope, the primary image is formed by an objective lens and enlarged by the optical lens to reveal a virtual image.
		A)	True
		B)	False


2	Microscope resolution increases as the wavelength of radiation used to illuminate the specimen decreases.
		A)	True
		B)	False


3	The fluorescence microscope has become an essential tool in medical microbiology to identify bacterial pathogens.
		A)	True
		B)	False


4	Objective lenses with higher magnification generally have a shorter working distance.
		A)	True
		B)	False


5	Even on the best light microscopes, increasing total magnification beyond 1500 cannot result in better resolution of the image.
		A)	True
		B)	False


6	Darkfield microscopy is best for visualizing live, motile microorganisms.
		A)	True
		B)	False


7	Fluorescence microscopy must be done on fixed and stained microorganisms.
		A)	True
		B)	False


8	Fluorescence microscopy may be used with specially stained antibodies to detect specific microorganisms.
		A)	True
		B)	False


9	Negative stains color the structures of interest a deep black.
		A)	True
		B)	False


10	Chromogenic stains which consist of a single dye rather than several dyes in combination are known as basic stains .
		A)	True
		B)	False


11	Transmission electron microscopy has a practical magnification of greater than 100,000x.
		A)	True
		B)	False


12	Acid fast stains interact with the waxy structures of the cell wall of Mycobacterium species.
		A)	True
		B)	False


13	Bacterial flagella are too small to be seen by light microscopy without staining the specimen.
		A)	True
		B)	False

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