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Animation Quiz 1 - Sanger Sequencing
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Review the animation and answer the questions that follow to test your understanding.

The primer used in Sanger sequencing
A)can have any nucleotide sequence
B)must have a sequence beginning and ending with the same nucleotide
C)has a nucleotide sequence complementary to the 3' end of the region to be copied
D)has a nucleotide sequence complementary to the 5' end of the region to be copied
To carry out Sanger sequencing a mixture is needed containing
A)single-stranded DNA
B)DNA polymerase
C)four deoxyribonucleotides A, T, C, G
D)all of the above
When a dideoxyribonucleotide is added to the tube
A)replication of the strand continues
B)replication of the strand stops
C)replication of the strand is not affected
D)replication of the strand is speeded up
To sequence DNA by this method, the DNA must first be made in single stranded form.
Examining the electrophoresis gel and reading from bottom to top, one base at a time, gives the sequence of DNA.

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