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Biology Laboratory Manual, 6/e

Darrell S. Vodopich, Baylor University
Randy Moore, University of Minnesota--Minneapolis

Exercise 15 - Molecular Biology and Biotechnology

Choose the one answer that best answers the question.


1	Biotechnology is also known as ___.
		A)	technology
		B)	genetic engineering
		C)	transformation
		D)	genetics


2	What is the process that directly manipulates an organism's DNA and genetic fate?
		A)	meiosis
		B)	genetic engineering
		C)	DNA isolation
		D)	mitosis


3	In this lab exercise, from which organism did you isolate DNA?
		A)	Escherichia coli
		B)	Bacillus megaterium
		C)	Halobacterium salinarum
		D)	Homo sapiens


4	DNA will precipitate out of solution in a test tube of lysed cells when ____ is added.
		A)	water
		B)	ethanol
		C)	acetone
		D)	acetic acid


5	DNA will begin to denature at a temperature of ____.
		A)	-40C
		B)	100C
		C)	37 to 44C
		D)	80 to 97C


6	What is the uptake and expression of DNA by a living cell?
		A)	genetic transformation
		B)	genetic engineering
		C)	biotechnology
		D)	competence


7	The host organism used in the genetic transformation experiment was ___.
		A)	Escherichia coli
		B)	Bacillus megaterium
		C)	Halobacterium salinarum
		D)	Homo sapiens


8	What carries a DNA sequence into a host?
		A)	plasmid
		B)	tRNA
		C)	mRNA
		D)	vector


9	The plasmid inserted into E. coli coded for ___.
		A)	ampicillin production
		B)	ampicillin resistance
		C)	DNA production
		D)	saline resistance


10	Halophilic refers to _____.
		A)	fat soluble
		B)	salt loving
		C)	salt soluble
		D)	bacteria

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