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Microbiology, Fifth Edition
Microbiology, 5/e
Lansing M Prescott, Augustana College
Donald A Klein, Colorado State University
John P Harley, Eastern Kentucky University
Recombinant DNA Technology

Chapter Objectives

After reading this chapter you should be able to:
  • discuss the use of recombinant DNA technology to genetically engineer various organisms
  • describe PCR and discuss its usefulness to genetic engineering and biotechnology
  • discuss the key role played by restriction endonucleases and DNA ligase in genetic engineering
  • discuss how plasmids, phages, cosmids, and artificial chromosomes are used as vectors for insertion and expression of foreign genes in an organism
  • discuss the use of both procaryotes and eucaryotes as target organisms for foreign gene insertion
  • discuss the contributions already made by the use of this technology
  • discuss the risks and the ethical problems associated with the use of this technology








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