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Microbiology, Fifth Edition
Microbiology, 5/e
Lansing M Prescott, Augustana College
Donald A Klein, Colorado State University
John P Harley, Eastern Kentucky University
Recombinant DNA Technology

Multiple Choice Quiz

Choose the best answer



1

The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content.
A)genetic engineering
B)population genetics
C)microbiology
2

Enzymes that recognize and cleave specific 4 to 8 base pair sequences
A)DNA ligase
B)helicases
C)restriction endonucleases
3

What is the normal role of these enzymes in bacterial cells?
A)to degrade the bacterial chromosome into small pieces during replication
B)to degrade invading phage DNA
C)to produce RNA primers for replication
4

Which type of restriction endonuclease cuts the DNA within the recognition site?
A)Type I
B)Type II
C)Type III
5

Bacterial cells protect their own DNA from degradation by restriction endonucleases
A)by methylating the DNA at the sites that the enzyme recognizes
B)by deleting all recognition sites from the genome
C)by not producing any restriction endonucleases
6

Vectors are:
A)molecules that degrade nucleic acids
B)molecules that are able to covalently bond to and carry foreign DNA into cells
C)molecules that protect host cells from invasion by foreign DNA
7

Separation of charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field
A)photoreactivation
B)gel electrophoresis
C)autoradiography
8

The Southern blotting technique depends on
A)similarities between the sequences of probe DNA and experimental DNA
B)the molecular mass of proteins
C)the amino acid sequence of a protein
9

A short (2-20 nucleotide) molecule
A)plasmid
B)vector
C)oligonucleotide
10

A molecular technique in which the DNA sequences between two oligonucleotide primers can be amplified
A)Southern blotting
B)Northern blotting
C)Polymerase Chain Reaction
11

The advantage to using DNA polymerases from thermophilic organisms in PCR is:
A)The DNA polymerases of these bacteria are much faster than those from other organisms
B)The DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
C)The DNA polymerases of these bacteria never make mistakes while replicating DNA
12

The enzyme used to covalently bond foreign DNA to a vector plasmid
A)DNA polymerase
B)restriction endonuclease
C)DNA ligase
13

A genomic library is:
A)a database where the sequence of an organisms genome is stored
B)a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
C)a book that describes how to isolate DNA from a particular organism
14

DNA that is obtained using processed mRNA molecules as a template
A)rDNA
B)mDNA
C)cDNA
15

For gene probes to be useful they must:
A)be large enough to contain gene-specific sequences
B)be labeled in some manner to allow detection
C)both of the above
16

Which of the following is not commonly used as vector?
A)artificial chromosome
B)cosmid
C)fungi
17

In genetic engineering, a chimera is:
A)an enzyme that links DNA molecules
B)a plasmid that contains foreign DNA
C)a virus that infects bacteria
18

Which of the following vector can maintain the largest fragment of foreign DNA?
A)YAC
B)cosmid
C)plasmid
19

An animal that has gained new genetic information from the acquisition of foreign DNA
A)a chimera
B)a transgenic animal
C)a vector
20

Electroporation is:
A)the process of separating charged molecules through a gel maintained in an electric field
B)the process of combining foreign DNA to an electrically charged vector molecule
C)the process of introducing DNA into cells by the application of high voltage pulses
21

The piece of equipment that introduces DNA into cells by via DNA-coated microprojectiles
A)laser
B)DNA probe
C)gene gun
22

Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by the use of these special cloning vectors
A)BACs
B)expression vectors
C)YACs
23

In which ways are recombinant DNA techniques are playing an important role in human health
A)helping to determine the molecular causes of disease
B)producing effective means to express proteins such as insulin and growth hormone
C)both of the above
24

Agrobacterium tumefaciens is:
A)a disease in humans that causes loss of sight
B)a bacterium that can be used to introduce DNA into plants
C)a fungi that is used to produce antibiotics in large amounts








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