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Microbiology, Fifth Edition
Microbiology, 5/e
Lansing M Prescott, Augustana College
Donald A Klein, Colorado State University
John P Harley, Eastern Kentucky University
Microbial Nutrition

Multiple Choice Quiz

Choose the best answer



1

All of the following are true about agar except it
A)liquefies at 100°C.
B)is a polysaccharide derived from a red alga.
C)Solidifies at approximately 40°C.
D)is metabolized by many bacteria.
E)facilitates obtaining pure cultures.

2.-4. Below are three different culture media. Use them to answer the following questions.

Medium AMedium BMedium C
Tributyrin (a lipid)GlucoseNa2HPO4
PO4PeptoneKH2PO4
KH2PO4(NH4)2SO4MgSO4
MgSO4KH2PO2CaCl2
(NH4)2SO4Na2HPO4NaHCO3



2

Which of these media, if any are chemically defined?
A)A
B)B
C)A and B
D)A and C
E)None of these
3

On which medium would an chemoorganotrophic heterotroph grow?
A)A
B)B
C)C
D)A and B
E)None of these
4

On which medium would an photolithotrophic autotroph grow?
A)A
B)B
C)C
D)A and C
E)None of these
5

If you are designing an experiment involving a photolithotrophic autotroph; in addition to light, which of the following would be most essential to maintaining growth of the organism?
A)a continual supply of abundant oxygen
B)a nutrient medium containing glucose
C)a source of CO2
D)keeping the cultures at 37°C
E)adding chlorophyll to the culture medium
6

If you are designing an experiment involving a chemolithotrophic autotroph; which of the following would be most essential to maintaining growth of the organism?
A)a nutrient medium containing glucose
B)a continual supply of abundant oxygen
C)keeping the cultures at 37°C
D)a source of reduced inorganic compound such as NH4
E)adding agar to the culture medium
7

The addition of which of the following would change a chemically defined medium into a complex medium?
A)Biotin (a vitamin)
B)K2HPO4
C)NH4NO3
D)Maltose
E)Yeast extract
8

Which of the following procedures can be used to isolate a pure culture of a bacterium from a mixture?
A)streak plating
B)dilution plating
C)enrichment culture
D)All the above can be used to isolate a pure culture of a bacterium from a mixture.
E)None of the above can be used to isolate a pure culture of a bacterium from a mixture.
9

Which of the following is a micronutrient?
A)Carbon
B)Manganese
C)Potassium
D)Magnesium
E)Nitrogen
10

Which of the following is a macronutrient?
A)Manganese
B)Magnesium
C)Molybdenum
D)Zinc
E)Cobalt
11

Which of the following uses radiant energy as their energy source?
A)Chemotroph
B)Lithotroph
C)Autotroph
D)Heterotroph
E)Phototroph
12

Which of the following obtain energy from the oxidation of inorganic or organic chemicals?
A)Chemotroph
B)Lithotroph
C)Autotroph
D)Heterotroph
E)Phototroph
13

An organism is capable of oxidizing H2S and using the energy obtained from the reaction to reduce carbon dioxide. Which type of metabolism does this organism possess?
A)Photolithotrophic autotroph
B)Chemoorganotrophic heterotroph
C)Photoorganotrophic heterotroph
D)Chemolithotrophic autotroph
E)Not enough information to determine which type
14

An organism is capable of oxidizing fatty acids to obtain energy, hydrogen, electrons and carbon. Which type of metabolism does this organism possess?
A)Photolithotrophic autotroph
B)Chemoorganotrophic heterotroph
C)Photoorganotrophic heterotroph
D)Chemolithotrophic autotroph
E)Not enough information to determine which type
15

An organism is capable of photosynthesis but use organic matter as their electron donor and carbon source. Which type of metabolism does this organism possess?
A)Photolithotrophic autotroph
B)Chemoorganotrophic heterotroph
C)Photoorganotrophic heterotroph
D)Chemolithotrophic autotroph
E)Not enough information to determine which type
16

An organism uses radiant energy as their energy source and carbon dioxide as their carbon source. Which type of metabolism does this organism possess?
A)Photolithotrophic autotroph
B)Chemoorganotrophic heterotroph
C)Photoorganotrophic heterotroph
D)Chemolithotrophic autotroph
E)Not enough information to determine which type
17

Addition of blood to a culture medium only allows the hemolytic bacteria that grow on the plate to be picked out. This is an example of a
A)Differential media.
B)Liquid media.
C)Chemically defined media.
D)Selective media.
E)Complex media.

For the next 2 items each item lists two categories, numbered I and II. Select the answers from the following key.



18

III
______Chemoorganotrophic heterotrophStaphylococcus
A)All members of I are also members of II, but not all members of II are members of I.
B)All members of II are also members of I, but not all members of I are members of II.
C)All members of I are members of II, and all members of II are members of I.
D)No member of I is also a member of II.
E)Some members of I are members of II and some are not, and some members of II are members of I and some are not.
19

III
______Nitrifying bacteriaChemolithotrophic autotroph
A)All members of I are also members of II, but not all members of II are members of I.
B)All members of II are also members of I, but not all members of I are members of II.
C)All members of I are members of II, and all members of II are members of I.
D)No member of I is also a member of II.
E)Some members of I are members of II and some are not, and some members of II are members of I and some are not.
20

III
______Gram negative bacteriaChemoorganotrophic heterotrop
A)All members of I are also members of II, but not all members of II are members of I.
B)All members of II are also members of I, but not all members of I are members of II.
C)All members of I are members of II, and all members of II are members of I.
D)No member of I is also a member of II.
E)Some members of I are members of II and some are not, and some members of II are members of I and some are not.
21

III
______Photoorganotrophic heterotrophChemolithotrophic autotroph
A)All members of I are also members of II, but not all members of II are members of I.
B)All members of II are also members of I, but not all members of I are members of II.
C)All members of I are members of II, and all members of II are members of I.
D)No member of I is also a member of II.
E)Some members of I are members of II and some are not, and some members of II are members of I and some are not.
22

III
______CyanobacteriaPhotolithotrophic autotroph
A)All members of I are also members of II, but not all members of II are members of I.
B)All members of II are also members of I, but not all members of I are members of II.
C)All members of I are members of II, and all members of II are members of I.
D)No member of I is also a member of II.
E)Some members of I are members of II and some are not, and some members of II are members of I and some are not.
23

III
______Photolithotrophic autotrophPlants
A)All members of I are also members of II, but not all members of II are members of I.
B)All members of II are also members of I, but not all members of I are members of II.
C)All members of I are members of II, and all members of II are members of I.
D)No member of I is also a member of II.
E)Some members of I are members of II and some are not, and some members of II are members of I and some are not.
24

III
______bacteria and archaebacteriaprokaryotic organism
A)All members of I are also members of II, but not all members of II are members of I.
B)All members of II are also members of I, but not all members of I are members of II.
C)All members of I are members of II, and all members of II are members of I.
D)No member of I is also a member of II.
E)Some members of I are members of II and some are not, and some members of II are members of I and some are not.
25

Vitamins are
A)organic compounds that are the building blocks of proteins.
B)organic molecules that are used for transfer of energy and information within the cell.
C)small organic molecules that make up a part of an enzyme cofactor.
D)a major energy source for bacteria when they are metabolized.
E)part of a nucleic acid.
26

A bacterial cell is analyzed for its elemental composition and determined to have a high concentration of potassium ions inside the cell relative to the outside. Several hours later another bacterium of the same strain is analyzed and determined to have the same distribution of potassium ions: high inside to low outside. Which of the following processes is involved in maintaining this ionic distribution?
A)passive diffusion
B)facilitated diffusion
C)active transport
D)endocytosis
E)exocytosis
27

Carrier proteins are involved in which of the following processes?
A)passive diffusion
B)facilitated diffusion
C)active transport
D)endocytosis
E)More than one of the above processes utilize carrier proteins.

28.-29. Compare the following entities (I and II).



28

I. The ability of ions and polar molecules to cross a membrane by passive diffusion.

II. The ability of lipophilic compounds to cross a membrane by passive diffusion

A)I is greater than II, mark A
B)I is less than II, mark B
C)I is exactly or approximately equal to II, mark C
D)I may stand in more than one of the above relations to II, mark D
29

I. The amount of energy expended passing an ion across a membrane utilizing active transport.

II. The amount of energy expended passing an ion across a membrane utilizing facilitated diffusion.

A)I is greater than II, mark A
B)I is less than II, mark B
C)I is exactly or approximately equal to II, mark C
D)I may stand in more than one of the above relations to II, mark D
30

Addition of salt to a culture medium only allows the salt-tolerant bacteria to grow. This is an example of a
A)Complex media.
B)Chemically defined media.
C)Enriched media.
D)Differential media.
E)Selective media.








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