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Foundations in Microbiology, 4/e
Kathleen Park Talaro, Pasadena City College
Arthur Talaro

Tools of the Laboratory: Methods for Studying Microorganisms

Supplemental Quiz

1. The labels have been accidentally removed from four (4) transmission electron micrographs of single cells. You know that one micrograph is of the Gram-negative bacterium E. coli, another is the Gram-positive Staphylococcus aureus, the third is the Gram-positive bacterium Bacillus cereus and the last is the bacterium Mycoplasm pneumoniae. Describe the mechanism to correctly re-label the micrographs.

2. Describe the use of a culture medium that (a) contains an agent that inhibits the growth of Gram-negative bacteria and (b) glows lime green in the presence of amino acids. What type of bacteria would be cultivated on this media? Defend your answer in 2-3 sentences.

3. Propose one mechanical and one culturing technique that would separate three bacteria mixed together in a soil sample. What conditions are required for successful isolations of pure cultures?