Review the animation and answer the questions that follow to test your understanding.

1			The direct ELISA test requires
			A)	known antibody
			B)	known antigen
			C)	complement
			D)	patient antibody
2			Place the following reactants in their proper order for the indirect ELISA test 1 = enzyme-linked antibody 2 = known antigen 3 = patient serum 4 = substrate
			A)	2 4 1 3
			B)	3 2 1 4
			C)	1 4 3 2
			D)	4 1 3 2
			E)	2 3 1 4
3			In the indirect ELISA test the enzyme-linked antibody will attach to
			A)	the patient antigen
			B)	the variable region of the patient antibody
			C)	the constant region of the patient antibody
			D)	the wall of the microtiter well
			E)	known antibody
4			In the indirect ELISA test the development of color means the patient has the antibody being tested for.
			A)	True
			B)	False
5			The direct ELISA test is used to test for the presence of specific antibodies in a patient's serum.
			A)	True
			B)	False