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Short Answer Quiz
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1

Why is the AT/GC rule critical for DNA replication?
2

Why was the use of nitrogen isotopes important for the success of Meselson and Stahl's experiment?
3

In Meselson and Stahl's experiment, what result would have supported the conservative model of DNA replication?
4

What is the primary function of the DnaA protein in bacterial DNA replication?
5

What is meant by bidirectional replication?
6

What is the size range of Okazaki fragments?
7

What is a processive enzyme?
8

Why are two ter sequences required in E. coli?
9

What proteins are found within the replisome?
10

Why must the lagging strand loop out of the replisome?
11

Describe exonuclease function and give two specific examples.
12

Some restriction enzymes which recognize 5'-GATC-3' sites are only able to cut the DNA if those sites are fully methylated. Other restriction enzymes which recognize this site are able to cut whether it is methylated or not. This is useful for molecular biologists who wish to control enzyme digestion when engineering new plasmids (see Chapter 18). In order to take advantage of this, they grow the plasmids in Dam- bacteria, which lack Dam methylase. However, these strains of bacteria tend to grow very poorly in culture. Why?
13

How could Kornburg's "in vitro DNA replication" system be used to identify all the proteins involved in DNA replication?
14

Why are lesion-replicating DNA polymerases useful for the eukaryotic cell?
15

In addition to possible problems with supercoiling, what problems might helicase have to overcome in eukaryotes in order to do its job?





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