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  1. Structural Overview of DNA Replication
    1. Existing DNA strands act as templates for the synthesis of new strands
    2. Three different models were proposed that described the net result of DNA replication
  2. Bacterial DNA Replication
    1. Bacterial chromosomes contain a single origin of replication
    2. Replication is initiated by the binding of DnaA protein to the origin of replication
    3. DNA strand separation and the synthesis of RNA primers are necessary before daughter strands of DNA can be made
    4. DNA polymerases link nucleotides to synthesize the daughter strands
    5. DNA polymerase III is a processive enzyme that uses deoxynucleoside triphosphates
    6. Replication is terminated when the replication forks meet at the terminus sequences
    7. Certain enzymes of DNA replication bind to each other to form a complex
    8. The fidelity of DNA replication is ensured by proofreading mechanisms
    9. Bacterial DNA replication is coordinated with cell division
    10. DNA replication can be studied in vitro
    11. The isolation of mutants has been instrumental in our understanding of DNA replication
  3. Eukaryotic DNA replication
    1. Initiation occurs at multiple origins of replication on linear eukaryotic chromosomes
    2. Eukaryotes contain several different DNA polymerases
    3. Nucleosomes containing new histone proteins are quickly formed after DNA replication
    4. The ends of eukaryotic chromosomes are replicated by telomerase





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