First Experiment

1		DNase I will digest a gene if
			A)	The DNA is tightly bound to histone proteins
			B)	The DNA is in an open conformation that is transcriptionally active
			C)	The DNA is highly compacted
			D)	The gene carries a nonsense mutation
2		In this experiment, the purpose of the sonication step was to
			A)	Denature the chromosomal DNA
			B)	Break open the cell nuclei
			C)	Break the DNA into small double-stranded fragments
			D)	Break open the bacterial cells
3		S1 nuclease cuts
			A)	Double-stranded DNA
			B)	Single-stranded DNA
			C)	Double-stranded RNA
			D)	Single-stranded RNA
4		When the probe was protected from S1 digestion, this means that
			A)	The probe is made from RNA.
			B)	The gene is transcriptionally active.
			C)	The probe is not binding to the gene.
			D)	The gene is compact and transcriptionally inactive.
5		In this experiment, if an excess amount of probe had been added compared to the amount of chromosomal DNA, how would the results have been affected?
			A)	Some of probe would be digested even if the gene was compact.
			B)	A smaller percentage of the probe would be digested.
			C)	The probe would promote transcriptional activation of the gene.
			D)	The results would have been the same.