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Study Outline
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  1. Sister Chromatid Exchange and Homologous Recombination
    1. The staining of harlequin chromosomes can reveal recombination between sister chromatids
    2. The holliday model describes a molecular mechanism for the recombination process
    3. More recent models have refined the molecular steps of homologous recombination
    4. Various proteins are necessary to facilitate homologous recombination
    5. Gene conversion may result from DNA gap repair synthesis or DNA mismatch repair
  2. Site-Specific Recombination
    1. The integration of viral genomes can occur by site-specific recombination
    2. Antibody diversity in the immune system is produced by site-specific recombination
  3. Transposition
    1. McClintock found that chromosomes of corn plants contain loci that can move
    2. Transposable elements and retroelements move via one of three transposition pathways
    3. Each type of transposable element has a characteristic pattern of DNA sequences
    4. Transposase catalyzes the excision and insertion of transposable elements
    5. Replicative transposition requires both transposase and resolvase
    6. Retroelements utilize reverse transcriptase and integrase for retrotransposition
    7. Transposons have become important tools in molecular biology





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