First Experiment

1		According to the hypothesis, the G. tetrahit species was created as
			A)	An allodiploid of G. speciosa and G. pubescens
			B)	An allotetraploid of G. speciosa and G. pubescens
			C)	An autotetraploid of G. speciosa
			D)	None of the above
2		The intermediate F2 strain was identified
			A)	Based on its phenotype that was intermediate between G. speciosa and G. pubescens
			B)	By karyotyping 200 offspring and luckily finding one that had two sets of chromosomes from G. speciosa and one set from G. pubescens
			C)	By treating the F1 allodiploids with colchicine
			D)	None of the above
3		When the intermediate F2 strain was crossed to G. pubescens, an "artificial" G. tetrahit was obtained because
			A)	A diploid gamete (due to nondisjunction) from G. pubescens united with a gamete from the intermediate F2 strain.
			B)	A normal gamete from G. pubescens united with a gamete from the intermediate F2 strain.
			C)	A gamete from the "artificial" G. tetrahit that was due to complete nondisjunction united with a normal gamete from G. pubescens.
			D)	None of the above
4		Evidence that the natural and artificial G. tetrahit were genetically similar was based on
			A)	Phenotype and the ability to produce viable offspring when crossed to each other
			B)	Phenotype only
			C)	The ability to produce viable offspring only
			D)	None of the above
5		If Müntzing had known about colchicine, how might he have modified his protocol to produce an "artificial" G. tetrahit?
			A)	Treat the intermediate F2 strain with high doses of colchicine and self fertilize
			B)	Treat G. speciosa with high doses of colchicine and self fertilize
			C)	Treat G. pubescens with high does of colchicine and self fertilize
			D)	Treat the F1 allodiploids with high doses of colchicine and self fertilize

Second Experiment

6		In this experiment, what is the purpose of PCR?
			A)	To clone different types of genes from extinct species
			B)	To amplify the 12S rRNA gene from extinct and modern species
			C)	To produce cDNA
			D)	To produce mRNA
7		The purpose of sequence alignment among the 12S rRNA genes is to
			A)	Determine if the genes are the same length
			B)	Determine where a particular gene begins
			C)	Determine the level of sequence similarity among genes
			D)	None of the above
8		Since Moa1 is evolutionarily closer to Moa2 compared to Kiwi2, we would expect the DNA sequence of the 12S rRNA gene from Moa1 to be
			A)	More similar to the 12S rRNA gene from Moa2
			B)	More similar to the 12S rRNA gene from Kiwi2
			C)	Less similar to the 12S rRNA gene from Moa2
			D)	None of the above
9		According to the alignment shown in the data, which two sequences are closest to each other?
			A)	Emu and cassowary
			B)	Moa4 and ostrich
			C)	Rhea1 and cassowary
			D)	Ostrich and cassowary
10		How might you verify that a PCR product from an extinct DNA sample is really an amplification of the extinct DNA and not an amplification of some DNA that might be contaminating the sample?
			A)	Determine its sequence
			B)	Use two or more extinct samples from different locations and see if you get the same DNA sequence
			C)	Use the same sample, but repeat the PCR multiple times and see if you get the same result.
			D)	None of the above.