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Short Answer Quiz
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1

Mutations are generally easier to identify in haploid organisms, because:
2

List the three mechanisms by which bacterial cells can transfer genetic material.
3

In the experiment by Lederberg and Tatum (illustrated in Fig 6.1), why were bacterial cells unable to grow unless the two strains were mixed together?
4

Describe the F factor.
5

What is the main difference between an Hfr and an F+ bacterial strain?
6

Why does Hfr conjugation usually not produce conjugation competent recipient strains?
7

What is the major difference between the lytic and lysogenic life cycles of bacteriophage?
8

What is the equation used to calculate map distance between genes mapped by cotransduction?
9

Two newly discovered genes in suburban bacteria, suv (suv- mutants require gasoline for survival) and socR (socR- mutants form colonies that resemble a soccer ball), are transmitted so closely together in interrupted mating experiments that it has been difficult to map them carefully. In a cotransduction experiment beginning with an suv- socR- recipient strain and using a phage that carries DNA pieces equal to about 2 minutes, of 542 suv+ colonies identified, 40 were also socR+. Calculate the distance between these two genes.
10

Which type(s) of genetic transfer is able to transfer extrachromosomal DNA into recipient bacteria?
11

Differentiate between horizontal and vertical gene transfer.
12

Why are bacteria found in hospitals more likely to be antibiotic resistant than those found elsewhere?
13

Describe the key structures of T4 bacteriophage.
14

In an intragenic mapping experiment, why is the number of wild type plaques doubled to calculate distance between the genes?





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