First Experiment

1		If the X-inactivation hypothesis had been incorrect such that both alleles of the G-6-PD gene were active in somatic cells, how would the results have been different?
			A)	Neither band would appear in the lanes from the cloned cells.
			B)	Both bands would appear in the lanes from the cloned cells.
			C)	The same results would have been obtained.
			D)	All of the cloned cells would have expressed the fast allele.
2		The purpose of plating the cells sparsely on solid growth media was to
			A)	Allow a single cell to grow into a colony of many cells
			B)	Turn on one of the G-6-PD alleles in the epithelial cells
			C)	Turn off one of the G-6-PD alleles in the epithelial cells
			D)	Promote faster growth
3		When plating sparsely onto the solid growth media, if two cells happened to be very close to each other, a single colony derived from both cells could express both alleles if
			A)	One original cell expressed the fast allele and the other original cell expressed the slow allele
			B)	If both of the original cells expressed the fast allele
			C)	If both of the original cells expressed the slow allele
			D)	None of the above
4		The reason why the two G-6-PD proteins migrate differently during gel electrophoresis is because
			A)	They are the products of totally different genes.
			B)	The fast allele is expressed at a higher level.
			C)	The slow allele is expressed at a higher level.
			D)	There is a small difference in the amino acid sequence between the two alleles that affects the size of the protein or its charge.
5		If cells were derived from an embryo prior to X inactivation (instead of from an adult female), the results would be different such that
			A)	Neither allele would be expressed.
			B)	Both alleles would be expressed.
			C)	Only one of the two alleles would be expressed.
			D)	None of the above