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cDNA
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Molecular Biology, 4/e
Robert F. Weaver
Molecular Cloning Methods
Genes into Plants Using the Ti-plasmid
View the animation below, then complete the quiz to test your knowledge of the concept.
1
Cloned genes are first inserted within the _____ to generate a transgenic plant.
A)
the Agrobacterium genome
B)
the plant genome
C)
the Ti plasmid
D)
chloroplasts of the plant
2
The cloned gene of interest is inserted where in the Ti plasmid?
A)
just prior to the T-DNA
B)
within the T-DNA
C)
anywhere in the plasmid
D)
cloned genes are not inserted into the plasmid
3
Using this procedure, it is possible to produce a transgenic plant that contains the gene of interest ________.
A)
in the original cell only
B)
in the tumors
C)
in all of the cells of the plant
D)
none of the above
4
In the natural world, the T-DNA of the Ti plasmid is used to form a tumor on a plant.
A)
True
B)
False
5
To form a transgenic plant, researchers would select those plants that have had tumors created by an Agrobacterium tumefaciens infection.
A)
True
B)
False
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