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Molecular Biology, 4/e
Robert F. Weaver

Molecular Cloning Methods

Genes into Plants Using the Ti-plasmid

View the animation below, then complete the quiz to test your knowledge of the concept.



1

Cloned genes are first inserted within the _____ to generate a transgenic plant.
A)the Agrobacterium genome
B)the plant genome
C)the Ti plasmid
D)chloroplasts of the plant
2

The cloned gene of interest is inserted where in the Ti plasmid?
A)just prior to the T-DNA
B)within the T-DNA
C)anywhere in the plasmid
D)cloned genes are not inserted into the plasmid
3

Using this procedure, it is possible to produce a transgenic plant that contains the gene of interest ________.
A)in the original cell only
B)in the tumors
C)in all of the cells of the plant
D)none of the above
4

In the natural world, the T-DNA of the Ti plasmid is used to form a tumor on a plant.
A)True
B)False
5

To form a transgenic plant, researchers would select those plants that have had tumors created by an Agrobacterium tumefaciens infection.
A)True
B)False