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cDNA
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Molecular Biology, 4/e
Robert F. Weaver
Molecular Cloning Methods
Polymerase Chain Reaction
View the animation below, then complete the quiz to test your knowledge of the concept.
1
PCR requires all of the following EXCEPT
A)
primers.
B)
DNA ligase.
C)
DNA polymerase.
D)
DNA of interest.
E)
deoxyrobinucleotides.
2
If you start with one double-stranded DNA molecule and you perform SIX cycles of PCR, how many double-stranded copies of the DNA will you have?
A)
6
B)
8
C)
16
D)
32
E)
64
3
The most likely source of the
Taq
polymerase used in PCR is a bacterium that lives in
A)
soil.
B)
arctic ice.
C)
hot vents.
D)
humans.
E)
plants.
4
PCR can be used to amplify DNA from any source.
A)
True
B)
False
5
During the PCR, the hydrogen bonds of the double-stranded DNA molecules are broken by the enzyme helicase.
A)
True
B)
False
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