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Biology Laboratory Manual, 6/e
Darrell S. Vodopich, Baylor University
Randy Moore, University of Minnesota--Minneapolis

Exercise 7 - Spectrophotometry

Complete the essay questions below and provide your answers as required by your instructor.



1

If you are given one sample of a substance that contains multiple compounds that you wish to measure, could you use spectrophotometry to measure them all? Describe the experiment, including all controls, that would be necessary.
2

If you wanted to measure a substance that absorbed light at wavelengths other than the visible wavelengths, could you use spectrophotometry? If so, then what changes would have to be made to the spectrophotometer, if any?
3

Why can it be said that the absorption spectrum of a molecule is it's 'fingerprint'?








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