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Biology Laboratory Manual, 6/e
Darrell S. Vodopich, Baylor University
Randy Moore, University of Minnesota--Minneapolis


Exercise 7 - Spectrophotometry

Choose the one answer that best answers the question.



1

The visible portion of the electromagnetic spectrum occurs between ___ nm and ___ nm.
A)1, 10
B)10, 300
C)400, 740
D)800, 1200
2

Which part of the spectrophotometer is adjusted to select the desired wavelength?
A)light source
B)filter
C)sample
D)photodetector
3

To establish a standard curve for a chemical, the spectrophotometer wavelength is set on which value?
A)the lowest wavelength possible
B)the highest wavelength possible
C)the wavelength at which the chemical's absorption value is the lowest
D)the wavelength at which the chemical's absorption value is the highest
4

A blank contains the ___ but not the dissolved chemical.
A)solvent
B)solute
C)filter
D)absorption spectrum
5

What is the function of the photodetector?
A)It adjusts the wavelength passing through the sample.
B)It adjusts the intensity of the light source.
C)It converts light energy into electrical energy.
D)It displays the amount of light absorbed in absorbance or transmittance values.
6

The blank used to calibrate the spectrophotometer for testing the absorbance spectrum of chlorophyll contained ___.
A)water
B)acetone
C)cobalt chloride
D)chlorophyll
7

Using a standard curve, if you know the abosorbance of an unknown sample, what else can be determined about the unknown?
A)the wavelength of maximum absorbance
B)the molecular weight of the sample
C)the concentration of the sample
D)the identity of the sample
8

Using the figure of the standard curve in the lab manual, if the concentration of a sample is 20 mg/mL, then roughly what is the absorbance?
A)0.8
B)0.8 lumens
C)0.8 lumens/mg
D)1.2
9

Basically, what is the path of light through a spectrophotometer?
A)meter, photodetector, filter, sample, light source
B)meter, filter sample, photodetector, light source
C)light source, filter, sample, photodetector, meter
D)light source, sample, filter, photodetector, meter
10

Basically, what is the function of an absorption spectrum?
A)It converts light energy into electrical energy.
B)It is a graph of a chemical relating the absorbance to wavelength.
C)It is a graph of a chemical relating the absorbance to concentration.
D)It is the amount of radiation retained by a sample.