Table of Contents: Case Study Exercise 1 Safety Considerations in the Microbiology Laboratory Case Study Exercise 2 Microscopy and Measurement of Microscopic Specimens Case Study Exercise 3 Identification and Classification of Algae Case Study Exercise 4 Survey of Medically Important Protozoa Case Study Exercise 5 Identification and Classification of Fungi Case Study Exercise 6 Ubiquity of Microorganisms Case Study Exercise 7 Aseptic and Pure Culture Techniques Case Study Exercise 8 Simple Staining, Negative Staining, and Gram Staining Case Study Exercise 9 Capsular Staining Case Study Exercise 10 Endospore Staining Case Study Exercise 11 Acid-Fast Staining Case Study Exercise 12 Viable Plate Count Case Study Exercise 13 Cultivation of Anaerobes Case Study Exercise 14 Temperature Effects on Bacterial Growth and Survival Case Study Exercise 15 pH and Microbial Growth Case Study Exercise 16 Effects of Osmotic Pressure on Bacterial Growth Case Study Exercise 17 Lethal Effects of Ultraviolet Light Case Study Exercise 18 Evaluation of Disinfectants: Use-Dilution Method Case Study Exercise 19 Effectiveness of Hand Scrubbing Case Study Exercise 20 Antimicrobic Sensitivity Testing: Kirby-Bauer, Tube Dilution, and E-Test Methods Case Study Exercise 21 Phage Typing of Bacteria Case Study Exercise 22 Simulated Epidemic Case Study Exercise 23 Morbidity and Mortality Weekly Report Case Study Exercise 24 Bacterial Transformation Case Study Exercise 25 The Ames Test Case Study Exercise 26 DNA Extraction from Bacterial Cells Case Study Exercise 27 DNA Profiling Case Study Exercise 28 Measures of Water Quality: Most Probable Number Procedure Case Study Exercise 29 Measures of Water Quality: Membrane Filtration Method Case Study Exercise 30 Measures of Milk Quality: Methylene Blue Reductase Test Case Study Exercise 31 Bacterial Counts of Food Case Study Exercise 32 Epidemiology of Gastrointestinal Illness: Differentiation of Enterobacteriaceae Case Study Exercise 33 Isolation and Identification of Staphylococci Case Study Exercise 34 Epidemiology of Gastrointestinal Illness: Differentiation of Enterobacteriaceae Case Study Exercise 35 Blood Typing Case Study Exercise 36 Differential White Blood Cell Count Case Study Exercise 37 Slide Agglutination Case Study Exercise 38 Enzyme Linked Immunosorbent Assay (ELISA) Case Study Exercise 39 Identification of Bacterial Unknowns Exercise 40 Colony Morphology Exercise 41 Growth in Solid and Liquid Media Exercise 42 Motility Methods: Wet Mount and Hanging Drop Exercise 43 Flagella Stain Exercise 44 Streak-Plate Isolation Exercise 45 Loop Dilution Exercise 46 Spread-Plate Exercise 47 Fluid Thioglycollate Medium Exercise 48 Mannitol Salt Agar Exercise 49 MacConkey Agar Exercise 50 Desoxycholate Agar Exercise 51 Endo Agar Exercise 52 Eosin Methylene Blue Agar Exercise 53 Hektoen Enteric Agar Exercise 54 Xylose Lysine Desoxycholate Agar Exercise 55 Blood Agar Exercise 56 Motility Media Exercise 57 SIM Medium Exercise 58 Kligler’s Iron Agar Exercise 59 Triple Sugar Iron Agar Exercise 60 Lysine Iron Agar Exercise 61 Litmus Milk Exercise 62 Oxidation-Fermentation Tes Exercise 63 Phenol Red Broth Exercise 64 Purple Broth Exercise 65 Methyl Red and Voges-Proskauer Tests Exercise 66 Catalase Test Exercise 67 Oxidase Test Exercise 68 Nitrate Reduction Test Exercise 69 Coagulase Test Exercise 70 Citrate Test Exercise 71 Malonate Test Exercise 72 Decarboxylation Test Exercise 73 Phenylalanine Deaminase Test Exercise 74 Bile Esculin Test Exercise 75 Starch Hydrolysis Exercise 76 ONPG Test Exercise 77 Urease Test Exercise 78 Casease Test Exercise 79 Gelatinase Test Exercise 80 DNase Test Exercise 81 Lipase Test Exercise 82 CAMP Test Exercise 83 PYR Test Exercise 84 API 20E System Exercise 85 Enterotube II System Exercise 86 Antibiotic Disk Sensitivity Tests Exercise 87 b-Lactamase Test Exercise 88 Viable Plate Count Exercise 89 Direct Cell Count
Appendix A: Spectrophotometric Determination of Bacterial Growth: Use of the Spectrophotometer
Appendix B: Use of Pipettes in the Laboratory
Appendix C: Preparation of Culture Media
Appendix D: Media, Reagents, and Stain Formulas
Appendix E: Data Sheet for Unknown Identification
Glossary
Credits
Index
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2015